KbvR mutant of Klebsiella pneumoniae affects the synthesis of type 1 fimbriae and provides protection to mice as a live attenuated vaccine.
KbvR mutant of Klebsiella pneumoniae affects the synthesis of type 1 fimbriae and provides protection to mice as a live attenuated vaccine.
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肺炎克雷伯菌的 KbvR 突变体影响 1 型菌毛的合成，并作为减毒活疫苗为小鼠提供保护
DOI：
10.1186/s13567-022-01116-y
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发表时间：
2022-11-26
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
肺炎克雷伯氏菌是人类和动物严重感染的主要原因，多重耐药菌株的出现突出了开发有效疫苗预防此类感染的必要性。减毒活疫苗是兽医领域有吸引力的候选疫苗。我们最近的特征在于，K。肺炎克雷伯菌kbvR（Klebsiella biofilm and virtual regulator）突变株在小鼠模型中是高度减毒株。在本研究中，评价ΔkbvR株作为减毒活疫苗的特性、安全性和保护效力。在ΔkbvR菌株中，1型菌毛的合成和活性增加。通过皮下途径接种105、106和107个菌落形成单位（CFU）剂量的ΔkbvR菌株的所有小鼠均存活。用105或107 CFU ΔkbvR两种剂量皮下免疫引起了强烈的体液免疫应答，并提供了对以下K.肺炎性腹膜内感染。105 CFU剂量免疫的小鼠抗血清不仅对同种型菌株而且对不同血清型菌株的调理吞噬能力和补体介导的溶解作用均有提高。从105 CFU剂量免疫的小鼠的抗血清的被动转移提供了对K.肺炎感染。总的来说，我们的研究结果表明ΔkbvR菌株作为一种新的抗克雷伯氏菌疫苗候选者的巨大潜力。肺炎感染的动物或人类。
Klebsiella pneumoniae is a leading cause of severe infections in humans and animals, and the emergence of multidrug-resistant strains highlights the need to develop effective vaccines for preventing such infections. Live attenuated vaccines are attractive vaccine candidates available in the veterinary field. We recently characterized that the K. pneumoniae kbvR (Klebsiella biofilm and virulence regulator) mutant was a highly attenuated strain in the mice model. In the present study, the characterization, safety, and protective efficacy of ΔkbvR strain as a live attenuated vaccine were evaluated. The synthesis and activity of type 1 fimbriae were increased in the ΔkbvR strain. All mice inoculated by the subcutaneous route with 105, 106, and 107 colony-forming units (CFU) doses of the ΔkbvR strain survived. Subcutaneous immunization with two doses of 105 or 107 CFU ΔkbvR elicited a robust humoral immune response, and provided protection against the following K. pneumoniae intraperitoneal infection. The antisera of mice immunized with 105 CFU dose improved the opsonophagocytic ability and complement-mediated lysis not only to the same serotype strain but also to the different serotype strain. The passive transfer of antisera from 105 CFU dose-immunized mice provided protection against K. pneumoniae infection. Overall, our results suggest the great potential of the ΔkbvR strain as a novel vaccine candidate against K. pneumoniae infections in herds or humans.
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影响因子：
4.8
作者：
Banerjee K;Motley MP;Diago-Navarro E;Fries BC
通讯作者：
Fries BC
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影响因子：
4.4
作者：
Hu G;Chen X;Chu W;Ma Z;Miao Y;Luo X;Fu Y
通讯作者：
Fu Y
影响因子：
12.2
作者：
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通讯作者：
Brisse S
影响因子：
12.8
作者：
Lee WH;Choi HI;Hong SW;Kim KS;Gho YS;Jeon SG
通讯作者：
Jeon SG
影响因子：
2.8
作者：
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通讯作者：
Unden, G