Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae.
Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae.
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外膜磷酸蛋白作为针对肺炎克雷伯氏菌的疫苗的免疫原性。
DOI：
10.1186/s13567-022-01023-2
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发表时间：
2022-01-21
影响因子：
4.4
通讯作者：
Fu Y
中科院分区：
文献类型：
--
作者：
Hu G;Chen X;Chu W;Ma Z;Miao Y;Luo X;Fu Y
近年来，肺炎克雷伯氏菌（Klebsiella pneumoniae，KP）在不同动物中引起疾病暴发，造成严重的经济损失和生物安全问题。考虑到KP广泛的抗生素耐药性，疫苗是对抗感染的最有效工具。然而，在兽医领域仍然没有可用的KP疫苗。我们的研究结果表明，高度保守的外膜磷酸通道蛋白（PhoE）的KP是免疫原性的小鼠和elevening高滴度的抗体，被证明是特异性的PhoE通过免疫印迹。用PhoE免疫也诱导了稳健的细胞介导的免疫，并引起高水平的IFN-γ和IL-4的分泌，表明诱导了混合的Th 1和Th 2应答。PhoE免疫小鼠的血清比PBS对照小鼠的血清诱导显著更高的补体介导的KP细胞裂解。最后，用PhoE免疫的小鼠显著保护免于KP攻击，具有更好的存活率和减少的内脏细菌负荷。我们的数据强调了PhoE作为KP感染疫苗的新型候选抗原的巨大潜力。在线版本包含补充材料，可通过10.1186/s13567-022-01023-2获得。
In recent years, Klebsiella pneumoniae (KP) has caused disease outbreaks in different animals, resulting in serious economic losses and biosafety concerns. Considering the broad antibiotic resistance of KP, vaccines are the most effective tools against infection. However, there is still no KP vaccine available in the veterinary field. Our results indicate that the highly conserved outer membrane phosphoporin (PhoE) of KP is immunogenic in mice and elicits high titers of antibodies that were shown to be specific for PhoE by immunoblotting. Immunization with PhoE also induced robust cell-mediated immunity and elicited the secretion of high levels of IFN-γ and IL-4, suggesting the induction of mixed Th1 and Th2 responses. Sera from PhoE-immunized mice induced significantly higher complement-mediated lysis of KP cells than did sera from the PBS control mice. Finally, mice immunized with PhoE were significantly protected against KP challenge, with better survival and a reduced visceral bacterial load. Our data underscore the great potential of PhoE as a novel candidate antigen for a vaccine against KP infection. The online version contains supplementary material available at 10.1186/s13567-022-01023-2.
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影响因子：
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影响因子：
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