Histotripsy fractionation of prostate tissue: local effects and systemic response in a canine model.
Histotripsy fractionation of prostate tissue: local effects and systemic response in a canine model.
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DOI：
10.1016/j.juro.2010.11.044
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Roberts WW
中科院分区：
文献类型：
--
作者：
Hempel CR;Hall TL;Cain CA;Fowlkes JB;Xu Z;Roberts WW
组织破坏术是一种体外超声（US）技术，利用空化机制产生非热组织破坏。以前，我们证明了组织碎石术用于分离和立即减积前列腺组织的可行性。本研究的目的是表征体内犬模型中前列腺组织组织粉碎治疗后的局部效应和全身反应。在全身麻醉下，对18只完整的雄性犬受试者经腹进行前列腺组织碎石术。从高度聚焦的750 kHz压电超声换能器（15 cm孔径，3×3×8 mm焦点体积）以300 Hz脉冲重复率输送声脉冲（4微秒）。在组织破坏后的规定时间点（0、7、28或56天）收获前列腺和周围结构。定期评估血液和尿液参数，同时每天进行包含经验证兽医疼痛量表的临床评价。传统的经直肠超声成像便于靶向的焦点体积，并提供实时评估的空化活动。目标体积的分离和排尿产生的碎片的清除在每个前列腺内产生治疗腔。在治疗后28天内，未观察到声学附带损伤，治疗腔发生尿路上皮化。治疗后仅观察到一过性实验室异常和极轻微血尿。疼痛评分仅显示轻度治疗后不适。组织碎石术产生了一致的组织分离和前列腺减积，没有附带的声学损伤或临床副作用，并且在犬模型中耐受良好。
Histotripsy is an extracorporeal ultrasound (US) technology that utilizes cavitational mechanisms to produce non-thermal tissue destruction. Previously, we demonstrated the feasibility of histotripsy for fractionation and immediate debulking of prostate tissue. The purpose of this study is to characterize the local effects and systemic response after histotripsy treatment of prostate tissue in an in-vivo canine model. Histotripsy was applied transabdominally to the prostate in eighteen intact male canine subjects under general anesthesia. Acoustic bursts (4 microseconds) were delivered at 300 Hz pulse repetition rate from a highly focused 750 kHz piezoelectric US transducer (15 cm aperture, 3×3×8 mm focal volume). The prostate and surrounding structures were harvested at prescribed time points (0, 7, 28, or 56 days) following histotripsy. Blood and urine parameters were assessed periodically while clinical evaluation incorporating a validated veterinary pain scale was performed daily. Conventional transrectal US imaging facilitated targeting of the focal volume and provided real-time assessment of cavitation activity. Fractionation of the targeted volume and clearance of the resultant debris with urination produced a treatment cavity within each prostate. No acoustic collateral damage was seen and urothelialization of the treatment cavity occurred within 28 days of treatment. Only transient lab abnormalities and minimal hematuria were noted after treatment. Pain scores revealed only mild post treatment discomfort. Histotripsy produced consistent tissue fractionation and prostate debulking without collateral acoustic injury or clinical side effects and was well tolerated in the canine model.
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影响因子：
2.4
作者：
Xu, Z;Fowlkes, JB;Cain, CA
通讯作者：
Cain, CA
影响因子：
23.4
作者：
Ahyai, Sascha A.;Lehrich, Karin;Kuntz, Rainer M.
通讯作者：
Kuntz, Rainer M.
DOI：
10.1109/tuffc.2003.1244746
发表时间：
2003-10-01
影响因子：
3.6
作者：
Tran, BC;Seo, J;Cain, CA
通讯作者：
Cain, CA
影响因子：
3.4
作者：
Tunuguntla, HSGR;Evans, CP
通讯作者：
Evans, CP
影响因子：
6.6
作者：
Wei, JT;Calhoun, E;Jacobsen, SJ
通讯作者：
Jacobsen, SJ