Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation.
Integrated PPI- and WGCNA-retrieval of hub gene signatures for soft substrates inhibition of human fibroblasts proliferation and differentiation.
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DOI：
10.18632/aging.204258
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发表时间：
2022-09-02
期刊：
影响因子：
5.2
通讯作者：
Li, Yulin
中科院分区：
文献类型：
--
作者：
Xu, Ziran;Zhou, Tian;Wang, Yin;Zhu, Leijie;Tu, Jihao;Xu, Zhixiang;Li, Lisha;Li, Yulin
成纤维细胞是创伤修复过程中最重要的功能细胞，其功能可被病理部位的不同信号激活。尽管伤口修复与微环境僵硬有关，但基质僵硬对FBS的影响仍然难以捉摸。在本研究中，使用转化生长因子-β1模拟病理条件下的纤维化环境。我们发现，与组织培养塑料相比，软基质使FBS变得细长，主要改变的生物学功能是抑制增殖和分化能力。通过PPI和WGCNA分析，发现63个HUB基因，包括GADD45A、CDKN3、HIST2H3PS2、ACTB等，可能是软底物影响FBS增殖分化的主要靶点。我们的研究结果不仅为基质僵硬对人皮肤FBS功能的影响提供了更详细的报道，而且可能为改善因细胞过度增殖引起的瘢痕等疾病提供新的干预思路，具有潜在的临床应用前景。
Fibroblasts (FBs) are the most important functional cells in the process of wound repair, and their functions can be activated by different signals at the pathological site. Although wound repair is associated with microenvironmental stiffness, the effect of matrix stiffness on FBs remains elusive. In this study, TGF-β1 was used to mimic the fibrotic environment under pathological conditions. We found that the soft substrates made FBs slender compared with tissue culture plastic, and the main altered biological function was the inhibition of proliferation and differentiation ability. Through PPI and WGCNA analysis, 63 hub genes were found, including GADD45A, CDKN3, HIST2H3PS2, ACTB, etc., which may be the main targets of soft substrates affecting the proliferation and differentiation of FBs. Our findings not only provide a more detailed report on the effect of matrix stiffness on the function of human skin FBs, but also may provide new intervention ideas for improving scars and other diseases caused by excessive cell proliferation, with potential clinical application prospects.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.3109/08977194.2011.595714
发表时间：
2011-10
期刊：
Growth factors (Chur, Switzerland)
影响因子：
--
作者：
Biernacka A;Dobaczewski M;Frangogiannis NG
通讯作者：
Frangogiannis NG
影响因子：
6.5
作者：
Achterberg, Volker F.;Buscemi, Lara;Hinz, Boris
通讯作者：
Hinz, Boris
影响因子：
2.4
作者：
Berman, Brian;Maderal, Andrea;Raphael, Brian
通讯作者：
Raphael, Brian
影响因子：
5
作者：
Bai, Xiaobin;Wang, Jia;Wang, Maode
通讯作者：
Wang, Maode