Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation.
Ginsenoside Rb1 Alleviates Alcohol-Induced Liver Injury by Inhibiting Steatosis, Oxidative Stress, and Inflammation.
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人参皂苷 Rb1 通过抑制脂肪变性、氧化应激和炎症来减轻酒精引起的肝损伤。
DOI：
10.3389/fphar.2021.616409
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发表时间：
2021
影响因子：
5.6
通讯作者：
Lv Z
中科院分区：
文献类型：
--
作者：
Lai Y;Tan Q;Xv S;Huang S;Wang Y;Li Y;Zeng T;Mo C;Chen Y;Huang S;Zhou C;Gao L;Lv Z
酒精性肝病（ALD）已成为全球健康的沉重负担。人参皂苷Rb 1（Ginsenoside Rb 1，GRb 1），从西洋参（Panax quinquefolium L.）GRb 1对多种疾病具有保护作用，但其在ALD中的作用及机制尚不清楚。本研究旨在探讨GRb 1对ALD的保护作用及其机制。将斑马鱼幼鱼暴露于350 mM乙醇中32 h，建立急性酒精性肝损伤模型，然后分别用6.25、12.5和25 μM GRb 1处理48 h。用100 mM乙醇刺激人肝细胞系，同时用6.25、12.5和25 μM GRb 1孵育24 h。油红O染色法、尼罗红染色法及甘油三酯测定法检测血脂变化。采用荧光探针法检测各组小鼠体内抗氧化能力，免疫组化、免疫荧光和实时荧光定量PCR法检测各组小鼠体内炎性细胞因子的表达水平。结果表明，GRb 1在体内的最佳浓度为12.5 μM时可减轻肝细胞中的脂质沉积。GRb 1逆转了酒精消耗引起的活性氧积累，并部分恢复了谷胱甘肽的水平。此外，GRb 1通过抑制肝实质中的中性粒细胞浸润并下调核因子-kappa B途径相关的促炎细胞因子（包括肿瘤坏死因子-α和白细胞介素-1 β）的表达来改善肝脏炎症。这项研究表明，GRb 1由于其抗脂质沉积以及抗氧化和抗炎作用，对酒精诱导的肝损伤具有保护作用。这些发现表明，GRb 1可能是一个有希望的候选人对ALD。
Alcoholic liver disease (ALD) has become a heavy burden on health worldwide. Ginsenoside Rb1 (GRb1), extracted from Panax quinquefolium L., has protective effects on many diseases, but the effect and mechanisms of GRb1 on ALD remain unknown. This study aimed to investigate the protective effects of GRb1 on ALD and to discover the potential mechanisms. Zebrafish larvae were exposed to 350 mM ethanol for 32 h to establish a model of acute alcoholic liver injury, and the larvae were then treated with 6.25, 12.5, or 25 μM GRb1 for 48 h. The human hepatocyte cell line was stimulated by 100 mM ethanol and meanwhile incubated with 6.25, 12.5, and 25 μM GRb1 for 24 h. The lipid changes were detected by Oil Red O staining, Nile Red staining, and triglyceride determination. The antioxidant capacity was assessed by fluorescent probes in vivo, and the expression levels of inflammatory cytokines were detected by immunohistochemistry, immunofluorescence, and quantitative real-time PCR. The results showed that GRb1 alleviated lipid deposition in hepatocytes at an optimal concentration of 12.5 μM in vivo. GRb1 reversed the reactive oxygen species accumulation caused by alcohol consumption and partially restored the level of glutathione. Furthermore, GRb1 ameliorated liver inflammation by inhibiting neutrophil infiltration in the liver parenchyma and downregulating the expression of nuclear factor-kappa B pathway-associated proinflammatory cytokines, including tumor necrosis factor-α and interleukin-1β. This study revealed that GRb1 has a protective effect on alcohol-induced liver injury due to its resistance to lipid deposition as well as antioxidant and anti-inflammatory actions. These findings suggest that GRb1 may be a promising candidate against ALD.
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影响因子：
5.6
作者：
Cornet C;Di Donato V;Terriente J
通讯作者：
Terriente J
影响因子：
39.3
作者：
Liu T;Zhang L;Joo D;Sun SC
通讯作者：
Sun SC
影响因子：
4.6
作者：
Kawaratani H;Tsujimoto T;Douhara A;Takaya H;Moriya K;Namisaki T;Noguchi R;Yoshiji H;Fujimoto M;Fukui H
通讯作者：
Fukui H
DOI：
10.1111/j.1440-1746.2007.05277.x
发表时间：
2008-03-01
影响因子：
4.1
作者：
De Minicis, Samele;Brenner, David A.
通讯作者：
Brenner, David A.
影响因子：
7.4
作者：
Janssen-Heininger, YMW;Poynter, ME;Baeuerle, PA
通讯作者：
Baeuerle, PA