blaKPC and rmtB on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient.
blaKPC and rmtB on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient.
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DOI：
10.1007/s10096-011-1481-x
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发表时间：
2012-07
影响因子：
4.5
通讯作者：
Li, L. -J.
中科院分区：
文献类型：
--
作者：
Sheng, J. -F.;Li, J. -J.;Tu, S.;Sheng, Z. -K.;Bi, S.;Zhu, M. -H.;Shen, X. -M.;Li, L. -J.
从我院神经外科同一患者中分离出具有多重耐药（MDR）模式的肠杆菌属（EA 76）和肺炎克雷伯菌（KP 76）。MDR K.肺炎也发生在这个部门。为了解这些分离株的耐药机制和分子流行病学特征，进行了包括抗菌药物敏感性测试、聚合酶链反应（PCR）、质粒分析、脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）的连续实验。EA 76和KP 76对除粘菌素和替加环素外的所有测试抗生素均具有耐药性。在两个分离株中鉴定了bla KPC-2、bla TEM-1、bla SHV-12、bla CTX-M-3、bla CTX-M-14和rmtB基因，其中bla KPC-2、bla TEM-1、bla CTX-M-14和rmtB在每个分离株中共携带在一个质粒上。进一步的分析显示两种携带KPC的质粒之间存在不同的限制性图谱。本次疫情中检出的11株碳青霉烯类耐药菌株对所有β-内酰胺类抗生素均耐药，其中63.64%（7/11）对氨基糖苷类抗生素耐药，72.73%（8/11）对喹诺酮类抗生素耐药。11株K. 7株氨基糖苷类抗生素耐药菌株的PFGE图谱与KP 76相同或仅一条带差异。此外，包括KP 76在内的8株氨基糖苷类耐药菌株均属于全国流行克隆ST 11。总体结果表明，E.稻瘟病菌属和ST 11 K.在我们的神经外科病房中，在单个质粒上共携带bla KPC和rmtB基因的肺炎。
Enterobacter amnigenus (EA76) and Klebsiella pneumoniae (KP76) isolates with multidrug-resistant (MDR) patterns were identified from the same patient in the neurosurgery department of our hospital. An outbreak of MDR K. pneumoniae had also occurred in this department. To characterize the resistance mechanism and molecular epidemiology of these isolates, sequential experiments including antimicrobial susceptibility testing, polymerase chain reaction (PCR), plasmid analysis, pulsed field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) were performed. EA76 and KP76 were resistant to all of the antibiotics tested, except colistin and tigecycline. bla KPC-2, bla TEM-1, bla SHV-12, bla CTX-M-3, bla CTX-M-14, and rmtB genes were identified in both isolates, with bla KPC-2, bla TEM-1, bla CTX-M-14, and rmtB being co-carried on one plasmid in each isolate. Further analysis showed different restriction patterns between the two KPC-carrying plasmids. Of the 11 carbapenem-resistant isolates found in the outbreak, all were resistant to all of the β-lactams tested, with 63.64% (7/11) also exhibiting resistance to aminoglycosides and 72.73% (8/11) exhibiting resistance to quinolones. PCR analysis and molecular typing of the 11 K. pneumoniae strains revealed that the seven aminoglycoside-resistant isolates shared the same antibiotic-resistant gene pattern and identical or one-band-difference PFGE profiles relative to KP76. In addition, all of the eight aminoglycoside-resistant isolates, including KP76, belonged to the national epidemic clone ST11. The overall results indicate the emergence of E. amnigenus and outbreak of ST11 K. pneumoniae, with both co-harboring bla KPC and rmtB genes on a single plasmid in our neurosurgery wards.
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影响因子：
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作者：
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通讯作者：
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DOI：
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