Continuously Monocropped Jerusalem Artichoke Changed Soil Bacterial Community Composition and Ammonia-Oxidizing and Denitrifying Bacteria Abundances.
Continuously Monocropped Jerusalem Artichoke Changed Soil Bacterial Community Composition and Ammonia-Oxidizing and Denitrifying Bacteria Abundances.
复制标题
DOI：
10.3389/fmicb.2018.00705
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Wu F
中科院分区：
文献类型：
--
作者：
Zhou X;Wang Z;Jia H;Li L;Wu F
在农业生态系统中，土壤微生物群落对植物的生长、营养和健康有着深远的影响。了解种植系统中的土壤微生物动态有助于确定农业实践如何影响微生物介导的土壤过程。在连续单作耶路撒冷（JA）的麦田中，对连续单作3年的JA系统土壤细菌群落进行了监测。土壤细菌群落组成估计的16 S rRNA基因的扩增子测序。氨氧化和反硝化细菌的丰度估计通过定量PCR分析的amoA，nirS和nirK基因。结果表明，单作1-2年的JA对土壤微生物α多样性影响不显著，第3茬JA降低了土壤微生物α多样性（P <0. 05）。主坐标分析和排列多变量方差分析表明，连续单作JA改变了土壤细菌群落结构和功能谱（P < 0.001）。在门的水平上，麦田中Latescibacteria、Planctomycetes和Cyanobacteria的相对丰度较高，第一茬JA以放线菌为主，第二茬JA以酸杆菌、Armatimonadetes、Gemmatimonadetes和Proteobacteria为主。在属的水平上，第一茬JA富集了具有病原拮抗和/或植物生长促进潜力的细菌物种，而在第二茬和第三茬JA中，包括潜在杀菌剂的属的成员增加。与木质纤维素降解和磷循环相关的KO基因相对丰度，第一季JA较高，第二季JA较高，第三季JA较高，第三季JA较高，第四季JA较高。JA第三季amoA基因丰度降低，nirK基因丰度升高，nirS基因丰度在JA第二季和第三季继续升高（P < 0.05）。冗余分析和Mantel检验发现，土壤有机碳和Olsen磷含量对土壤细菌群落的形成起着重要作用。总之，我们的研究结果表明，连续单作JA改变土壤细菌群落组成和功能潜力。
Soil microbial communities have profound effects on the growth, nutrition and health of plants in agroecosystems. Understanding soil microbial dynamics in cropping systems can assist in determining how agricultural practices influence soil processes mediated by microorganisms. In this study, soil bacterial communities were monitored in a continuously monocropped Jerusalem artichoke (JA) system, in which JA was successively monocropped for 3 years in a wheat field. Soil bacterial community compositions were estimated by amplicon sequencing of the 16S rRNA gene. Abundances of ammonia-oxidizing and denitrifying bacteria were estimated by quantitative PCR analysis of the amoA, nirS, and nirK genes. Results showed that 1–2 years of monocropping of JA did not significantly impact the microbial alpha diversity, and the third cropping of JA decreased the microbial alpha diversity (P < 0.05). Principal coordinates analysis and permutational multivariate analysis of variance analyses revealed that continuous monocropping of JA changed soil bacterial community structure and function profile (P < 0.001). At the phylum level, the wheat field was characterized with higher relative abundances of Latescibacteria, Planctomycetes, and Cyanobacteria, the first cropping of JA with Actinobacteria, the second cropping of JA with Acidobacteria, Armatimonadetes, Gemmatimonadetes, and Proteobacteria. At the genus level, the first cropping of JA was enriched with bacterial species with pathogen-antagonistic and/or plant growth promoting potentials, while members of genera that included potential denitrifiers increased in the second and third cropping of JA. The first cropping of JA had higher relative abundances of KO terms related to lignocellulose degradation and phosphorus cycling, the second cropping of JA had higher relative abundances of KO terms nitrous-oxide reductase and nitric-oxide reductase, and the third cropping of JA had higher relative abundances of KO terms nitrate reductase and nitrite reductase. The abundances of amoA genes decreased while nirK increased in the third cropping of JA, nirS continuously increased in the second and third cropping of JA (P < 0.05). Redundancy analysis and Mantel test found that soil organic carbon and Olsen phosphorus contents played important roles in shaping soil bacterial communities. Overall, our results revealed that continuous monocropping of JA changed soil bacterial community composition and its functional potentials.
登录
查看更多内容
DOI：
10.1099/ijs.0.65576-0
发表时间：
2008-05-01
影响因子：
2.8
作者：
Dastager, Syed G.;Lee, Jae-Chan;Kim, Chang-Jin
通讯作者：
Kim, Chang-Jin
影响因子：
4.8
作者：
Fierer, Noah;Bradford, Mark A.;Jackson, Robert B.
通讯作者：
Jackson, Robert B.
影响因子：
4.8
作者：
Bainard, Luke D.;Hamel, Chantal;Gan, Yantai
通讯作者：
Gan, Yantai
影响因子：
15.5
作者：
Fernandes AD;Reid JN;Macklaim JM;McMurrough TA;Edgell DR;Gloor GB
通讯作者：
Gloor GB
影响因子：
5.1
作者：
Coyotzi, Sara;Doxey, Andrew C.;Neufeld, Josh D.
通讯作者：
Neufeld, Josh D.