One-step glass-like coating of polycarbonate for seamless DNA purification and amplification on an integrated monolithic microdevice
One-step glass-like coating of polycarbonate for seamless DNA purification and amplification on an integrated monolithic microdevice
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一步式玻璃状聚碳酸酯涂层，用于在集成整体式微型设备上进行无缝 DNA 纯化和扩增
DOI：
10.1016/j.snb.2014.06.078
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发表时间：
2014
影响因子：
8.4
通讯作者：
N. Lee
中科院分区：
文献类型：
--
作者：
Yu Zhang;I. Yoo;N. Lee
我们提出了一种在室温下应用双[3-（三甲氧基硅基）丙基]氨基硅烷（ABPTMS）在聚碳酸酯（PC）表面实现玻璃状溶胶-凝胶涂层的新方法。ABPTMS是一种含仲胺基的氨基端硅烷偶联剂，可与PC的碳酸盐基团反应形成强聚氨酯键，不需要加热，也不需要对PC进行表面氧化。在本研究中，ABPTMS的硅烷醇基团自聚集在PC上形成了一层薄的玻璃状溶胶-凝胶层，这是PC表面形成溶胶-凝胶的唯一组分。所得到的溶胶-凝胶涂层还可以在相对温和的温度和大气压下在30分钟内通过形成硅氧烷键（sissingle bond或single bondSi）一步粘合两个PC衬底。通过剪切强度测试、高通量泄漏测试和分层测试来测量粘结强度，测得的最高粘结强度约为950 kPa。该粘合剂足够坚固，可以承受每分钟注入量几乎是所测试微通道总内部体积的2000倍的液体的强烈注入。作为实际应用，采用溶胶-凝胶包覆PC微设备进行DNA纯化，并与流式聚合酶链反应（PCR）单元集成，在单片PC设备上实现DNA的无缝纯化和同时扩增。230 b大肠杆菌DNA在70分钟内被成功纯化和扩增，简化了原本最繁琐和耗时的DNA分析操作。
We present a new method for realizing a glass-like, sol-gel coating on a polycarbonate (PC) surface by simply applying bis[3-(trimethoxysilyl)propyl]aminosilane (ABPTMS) at room temperature. ABPTMS, an amine-terminated silane coupling reagent bearing secondary amine groups, can form strong urethane bonds by reacting with the carbonate groups of PC, requiring neither heat nor surface oxidation of PC. In this study, a thin glass-like sol-gel layer was formed on PC via self-aggregation of the silanol groups of ABPTMS as the sole component for sol-gel formation on the PC surface. The resulting sol-gel coating also facilitated one-step bonding of two PC substrates by forming siloxane bonds (Sisingle bondOsingle bondSi) at a relatively mild temperature and under atmospheric pressure within 30 min. Bond strength was measured through shear strength tests, high-throughput leak tests, and delamination tests, and the highest measured bond strength was approximately 950 kPa. The bond was sufficiently robust to endure intense introduction of liquid with a per-minute injection volume that was nearly 2000 times the total internal volume of the microchannel tested. As a practical application, the sol-gel-coated PC microdevice was adopted for DNA purification, and was integrated with a flow-through polymerase chain reaction (PCR) unit to realize seamless DNA purification and amplification simultaneously on a monolithic PC device. 230 bpEscherichia coliDNA was successfully purified and amplified using the microdevice in 70 min, facilitating the otherwise most cumbersome and time-consuming operations of DNA analysis.
用红外振动回波光谱研究封装在硅溶胶-凝胶玻璃中的蛋白质的动力学。
影响因子：
15
作者：
Massari,AaronM;Finkelstein,IlyaJ;Fayer,MichaelD
通讯作者：
Fayer,MichaelD
影响因子：
6.1
作者：
Kricka, LJ;Fortina, P;Becker, H
通讯作者：
Becker, H