Highly Sensitive Thrombin Detection by Combination of Click Chemistry and Surface-Initiated Polymerization
Highly Sensitive Thrombin Detection by Combination of Click Chemistry and Surface-Initiated Polymerization
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通过结合点击化学和表面引发聚合进行高灵敏度凝血酶检测
DOI：
10.1149/2.0091915jes
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发表时间：
2019
影响因子：
3.9
通讯作者：
Jinming Kong
中科院分区：
文献类型：
--
作者：
Qianrui Liu;Dongxiao Wen;Lianzhi Li;Xueji Zhang;Jinming Kong
凝血酶与多种疾病有很强的相关性，提高凝血酶检测的敏感性具有重要意义。在此，我们报道了一种高灵敏度凝血酶检测（17 fM）的生物传感器，该传感器结合了点击化学和表面引发原子转移自由基聚合（SI-ATRP）。巯基修饰的适配体-1、凝血酶和叠氮标记的适配体-2之间形成三明治结构。然后通过电化学介导Cu (I)催化叠氮化物-炔环加成（eCuAAC）将SI-ATRP（丙炔基α-溴异丁酸酯，PBiB）引发剂引入电极表面。随后，基于抗坏血酸还原Mn （III）中位四（4-羧基苯基）卟啉（Mn (III)-TCPP）为Mn （II）中位四（4-羧基苯基）卟啉（Mn (II)-TCPP）的反应，SI-ATRP被激活，许多单体通过SI-ATRP接枝到电极表面。该方法的检出限提高了4.9× 10 3倍，灵敏度提高，具有良好的稳定性和重复性。
Thrombin has a strong correlation to a variety of diseases, and it is of great significance to improve the sensitivity of thrombin detection. Herein, we report a biosensor for highly sensitive thrombin detection (17 fM) by combination of click chemistry and surface-initiated atom transfer radical polymerization (SI-ATRP). A sandwich structure is formed among thiol-modified aptamer-1, thrombin and azido-labeled aptamer-2. Then the initiators of SI-ATRP (propargyl α-bromoisobutyrate, PBiB) can be introduced to electrode surface via electrochemically mediated Cu (I)-catalyzed azide-alkyne cycloaddition (eCuAAC). Subsequently, SI-ATRP is activated based on the reaction that ascorbic acid reduced Mn (III) meso-tetra (4-carboxyphenyl) porphine (Mn (III)-TCPP) to Mn (II) meso-tetra (4-carboxyphenyl) porphine (Mn (II)-TCPP), and numerous monomers are grafted to electrode surface via SI-ATRP. The limit of detection is enhanced about 4.9× 10 3-fold compared with previous strategy, the sensitivity of this biosensor can be improved and this method also have desirable stability and reproducibility.
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