Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection.
Transcriptome and Metabolome Analyses of Thitarodes xiaojinensis in Response to Ophiocordyceps sinensis Infection.
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DOI：
10.3390/microorganisms11092361
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发表时间：
2023-09-21
期刊：
影响因子：
4.5
通讯作者：
Meng Q
中科院分区：
文献类型：
--
作者：
Li M;Zhang J;Qin Q;Zhang H;Li X;Wang H;Meng Q
冬虫夏草在小金甲血中表现出超过5个月的营养生长。通过形态学观察和组学技术，阐明了中华水蛭独特的发育过程；然而，很少有关于宿主小金斑绦虫体内发生的变化的报道。RNA测序数据显示，当中华绒球孢子处于增殖阶段时，受感染幼虫脂肪体变化最大的是选择性上调的免疫识别和抗菌肽基因。当中华绒球孢子处于静止阶段时，小金绒球的免疫途径恢复到正常水平，这与中华绒球的成功定居相吻合。途径富集分析显示，能量代谢途径相关基因在这一阶段表达较高。代谢组学分析显示，侵染中华夜蛾后，血淋巴中氨基酸和脂质含量降低，而脂肪体中脂质含量升高。我们提出了首个整合转录组和代谢组学研究的小金银桃感染中华o.s。这将提高我们对宿主与昆虫病原真菌相互作用机制的理解，并为未来对这些相互作用中涉及的基因和途径的功能研究提供便利。
Ophiocordyceps sinensis exhibits more than 5 months of vegetative growth in Thitarodes xiaojinensis hemocoel. The peculiar development process of O. sinensis has been elucidated through morphological observation and omics technology; however, little information has been reported regarding the changes that occur in the host T. xiaojinensis. The RNA sequencing data showed that when O. sinensis blastospores were in the proliferative stage, the greatest change in the infected larval fat body was the selectively upregulated immune recognition and antimicrobial peptide genes. When O. sinensis blastospores were in the stationary stage, the immune pathways of T. xiaojinensis reverted to normal levels, which coincides with the successful settlement of O. sinensis. Pathway enrichment analysis showed a higher expression of genes involved in energy metabolism pathway in this stage. Metabolomic analyses revealed a reduction of amino acids and lipids in hemolymph, but an upregulation of lipids in the fat body of the host larvae after O. sinensis infection. We present the first transcriptome integrated with the metabolome study of T. xiaojinensis infected by O. sinensis. It will improve our understanding of the interaction mechanisms between the host and entomopathogenic fungi, and facilitate future functional studies of genes and pathways involved in these interactions.
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影响因子：
2.5
作者：
Baverstock, Jason;Roy, Helen E.;Pell, Judith K.
通讯作者：
Pell, Judith K.
DOI：
10.1615/intjmedmushr.v10.i3.30
发表时间：
2008-01-01
影响因子：
1.2
作者：
Holliday, John;Cleaver, Matt
通讯作者：
Cleaver, Matt
布鲁氏锥虫中的脂质液滴中的三酰基甘油储存。
影响因子：
3.7
作者：
Allmann S;Mazet M;Ziebart N;Bouyssou G;Fouillen L;Dupuy JW;Bonneu M;Moreau P;Bringaud F;Boshart M
通讯作者：
Boshart M
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL