SARS-CoV-2 replicates in respiratory ex vivo organ cultures of domestic ruminant species.
SARS-CoV-2 replicates in respiratory ex vivo organ cultures of domestic ruminant species.
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DOI：
10.1016/j.vetmic.2020.108933
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发表时间：
2021-01
影响因子：
3.3
通讯作者：
Lorusso A
中科院分区：
文献类型：
--
作者：
Di Teodoro G;Valleriani F;Puglia I;Monaco F;Di Pancrazio C;Luciani M;Krasteva I;Petrini A;Marcacci M;D'Alterio N;Curini V;Iorio M;Migliorati G;Di Domenico M;Morelli D;Calistri P;Savini G;Decaro N;Holmes EC;Lorusso A
利用EVOC研究了SARS-CoV-2在牛、羊和猪中的复制和嗜性。牛和羊的呼吸道组织能够维持病毒的复制，但猪的呼吸道组织不能。在S蛋白中携带突变D 614 G的SARS-CoV-2分离株具有更强的复制能力。SARS-CoV-2与牛和羊呼吸道表达ACE 2的细胞结合。有强有力的证据表明，2019年冠状病毒病（COVID-19）大流行的病原体严重急性呼吸综合征2型病毒（SARS-CoV-2）源自动物宿主。然而，确切的出现机制，所涉及的宿主物种，以及对家畜和农业动物的风险在很大程度上是未知的。一些家养动物物种，包括猫、雪貂和水貂，已被证明对SARS-CoV-2感染易感，而其他动物，如猪和鸡，则不易感染。重要的是，反刍动物对SARS-CoV-2的易感性尚不清楚，尽管它们经常与人类生活在一起。我们研究了复制和组织嗜性的两种不同的SARS-CoV-2分离物在三个农场动物物种的呼吸道-牛，羊，猪-使用呼吸离体器官培养物（EVOC）。我们证明，牛和羊的呼吸道组织，但不是猪，维持病毒复制在体外的两个分离株和SARS-CoV-2是相关的ACE 2表达细胞的呼吸道反刍动物物种。有趣的是，SARS-CoV-2分离物在刺突蛋白的位点614处含有氨基酸取代（突变D 614 G），该分离物在两种反刍动物物种的离体组织中以更高的幅度复制，支持先前使用人类细胞获得的结果。这些结果表明，额外的体内实验，涉及几个反刍动物物种是必要的，以确定其潜在的作用，这种病毒的流行病学。
Replication and tropism of SARS-CoV-2 in cattle, sheep, and pigs using EVOCs, were investigated. Respiratory tissues of cattle and sheep, but not those of pigs, are able to sustain viral replication. A SARS-CoV-2 isolate harbouring mutation D614 G in the S protein has greater replication capabilities. SARS-CoV-2 binds to ACE2-expressing cells of the respiratory tract of cattle and sheep. There is strong evidence that severe acute respiratory syndrome 2 virus (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic, originated from an animal reservoir. However, the exact mechanisms of emergence, the host species involved, and the risk to domestic and agricultural animals are largely unknown. Some domestic animal species, including cats, ferrets, and minks, have been demonstrated to be susceptible to SARS-CoV-2 infection, while others, such as pigs and chickens, are not. Importantly, the susceptibility of ruminants to SARS-CoV-2 is unknown, even though they often live in close proximity to humans. We investigated the replication and tissue tropism of two different SARS-CoV-2 isolates in the respiratory tract of three farm animal species - cattle, sheep, and pigs - using respiratory ex vivo organ cultures (EVOCs). We demonstrate that the respiratory tissues of cattle and sheep, but not of pigs, sustain viral replication in vitro of both isolates and that SARS-CoV-2 is associated to ACE2-expressing cells of the respiratory tract of both ruminant species. Intriguingly, a SARS-CoV-2 isolate containing an amino acid substitution at site 614 of the spike protein (mutation D614G) replicated at higher magnitude in ex vivo tissues of both ruminant species, supporting previous results obtained using human cells. These results suggest that additional in vivo experiments involving several ruminant species are warranted to determine their potential role in the epidemiology of this virus.
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影响因子：
13.2
作者：
Meekins DA;Morozov I;Trujillo JD;Gaudreault NN;Bold D;Carossino M;Artiaga BL;Indran SV;Kwon T;Balaraman V;Madden DW;Feldmann H;Henningson J;Ma W;Balasuriya UBR;Richt JA
通讯作者：
Richt JA
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
81.5
作者：
Matthay, Michael A.;Zemans, Rachel L.;Calfee, Carolyn S.
通讯作者：
Calfee, Carolyn S.
影响因子：
7.3
作者：
Hamming, I;Timens, W;van Goor, H
通讯作者：
van Goor, H
影响因子：
5.4
作者：
Gonzalez, Gaelle;Marshall, John F.;Murcia, Pablo R.
通讯作者：
Murcia, Pablo R.