MATLAB implementation of a dynamic clamp with bandwidth of >125 kHz capable of generating I Na at 37 °C.
MATLAB implementation of a dynamic clamp with bandwidth of >125 kHz capable of generating I Na at 37 °C.
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DOI：
10.1007/s00424-012-1186-8
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发表时间：
2013-04
影响因子：
4.5
通讯作者：
Cohen, Ira S.
中科院分区：
文献类型：
--
作者：
Clausen, Chris;Valiunas, Virginijus;Brink, Peter R.;Cohen, Ira S.
我们描述了利用高性能、低成本的标准家庭/办公室PC与高速(16位)数据采集模块接口的带宽>；125 kHz动态钳位的构造。高带宽是通过利用最近可用的软件进步(代码生成技术、优化的实时内核)实现的。利用可视化编程语言SIMULINK编制了动态夹具程序。膜电流的计算模块是用高级MatLab语言编写的，不需要用C语言编程。该仪器可用于单电池或双电池配置，具有在实验进行时修改程序的能力。我们描述了一种实时计算快瞬变Na+电流(INa)的算法，并使用适用于37°C的速率常数来测试其准确性和稳定性。然后，我们构建了一个程序，能够为细胞准备提供三种电流：INa、超极化激活的内向起搏器电流(IF)和内向整流性K+电流(IK1)。该程序可校正电极电流引起的IR降，并记录所有电压和电流。我们在双膜片钳HEK293细胞和电流钳制HEK293细胞上测试了该程序，在HEK293细胞中，动态钳位控制电流钳制放大器，电压钳制放大器控制膜电位，在电流钳制HEK293细胞中，动态钳制产生自发起搏行为，在其他被动细胞中表现出Na+尖峰。
We describe the construction of a dynamic clamp with bandwidth >125 KHz that utilizes a high performance, yet low cost, standard home/office PC interfaced with a high-speed (16 bit) data acquisition module. High bandwidth is achieved by exploiting recently available software advances (code-generation technology, optimized real-time kernel). Dynamic-clamp programs are constructed using Simulink, a visual programming language. Blocks for computation of membrane currents are written in the high-level matlab language; no programming in C is required. The instrument can be used in single- or dual-cell configurations, with the capability to modify programs while experiments are in progress. We describe an algorithm for computing the fast transient Na+ current (INa) in real time, and test its accuracy and stability using rate constants appropriate for 37°C. We then construct a program capable of supplying three currents to a cell preparation: INa, the hyperpolarizing-activated inward pacemaker current (If), and an inward-rectifier K+ current (IK1). The program corrects for the IR drop due to electrode current flow, and also records all voltages and currents. We tested this program on dual patch-clamped HEK293 cells where the dynamic clamp controls a current-clamp amplifier and a voltage-clamp amplifier controls membrane potential, and current-clamped HEK293 cells where the dynamic clamp produces spontaneous pacing behavior exhibiting Na+ spikes in otherwise passive cells.
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DOI：
10.1152/ajpendo.00194.2002
发表时间：
2003-07-01
影响因子：
5.1
作者：
Fridlyand, LE;Tamarina, N;Philipson, LH
通讯作者：
Philipson, LH
影响因子：
3
作者：
ROBINSON, HPC;KAWAI, N
通讯作者：
KAWAI, N
影响因子：
3.8
作者：
Verheijck, EE;Wilders, R;Joyner, RW;Golod, DA;Kumar, R;Jongsma, HJ;Bouman, LN;van Ginneken, ACG
通讯作者：
van Ginneken, ACG
影响因子：
17.1
作者：
Lu Z;Wu CY;Jiang YP;Ballou LM;Clausen C;Cohen IS;Lin RZ
通讯作者：
Lin RZ
影响因子：
4.6
作者：
Butera, RJ;Wilson, CG;Smith, JC
通讯作者：
Smith, JC