Effect of DMPS and DMSA on the placental and fetal disposition of methylmercury.
Effect of DMPS and DMSA on the placental and fetal disposition of methylmercury.
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DOI：
10.1016/j.placenta.2009.06.005
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发表时间：
2009-09
期刊：
影响因子：
3.8
通讯作者：
Zalups, R. K.
中科院分区：
文献类型：
--
作者：
Bridges, C. C.;Joshee, L.;Zalups, R. K.
甲基汞（CH3Hg+）是一种严重的环境毒物。怀孕期间接触这种金属可能会导致发育中的胎儿出现严重的神经和发育缺陷。令人惊讶的是，人们对汞离子穿过胎盘的机制知之甚少。尽管已表明 2,3-二巯基丙烷-1-磺酸盐 (DMPS) 和 2,3-二巯基丁二酸 (DMSA) 能够从各种器官和细胞中提取汞离子，但没有证据表明它们能够在母体接触 CH3Hg+ 后从胎盘或胎儿组织中提取汞。因此，本研究的目的是评估母体接触 CH3Hg+ 后 DMPS 和 DMSA 从胎盘和胎儿组织中提取汞离子的能力。怀孕的 Wistar 大鼠在怀孕第 11 天或第 17 天暴露于含有 [203Hg] 的 CH3HgCl，并在 24 小时后用盐水、DMPS 或 DMSA 处理。暴露于 CH3HgCl 后 48 小时收获母体器官、胎儿和胎盘。母体器官和组织中汞离子的分布与我们实验室之前报道的相似。胎盘和胎儿中汞离子的分布似乎取决于胎儿的孕龄。胎儿和胎盘的汞负荷随着胎儿年龄的增加而增加，DMPS 和 DMSA 可降低汞负荷，其中 DMPS 更有效。对妊娠第 19 天收获的胎儿的肝脏、总肾脏质量和大脑中的汞分布进行了检查。以每克组织为基础，在胎儿的总肾脏质量中检测到的汞含量最多，其次是大脑和肝脏。 DMPS 和 DMSA 减少了肝脏和大脑中的汞负荷，而只有 DMPS 对肾脏总质量有效。目前的研究结果首次表明，DMPS 和 DMSA 不仅能够从母体组织中提取汞离子，而且还能从母体接触 CH3Hg+ 后的胎盘和胎儿组织中提取汞离子。
Methylmercury (CH3Hg+) is a serious environmental toxicant. Exposure to this metal during pregnancy can cause serious neurological and developmental defects in a developing fetus. Surprisingly, little is known about the mechanisms by which mercuric ions are transported across the placenta. Although it has been shown that 2,3-dimercaptopropane-1-sulfonate (DMPS) and 2,3-dimercaptosuccinic acid (DMSA) are capable of extracting mercuric ions from various organs and cells, there is no evidence that they are able to extract mercury from placental or fetal tissues following maternal exposure to CH3Hg+. Therefore, the purpose of the current study was to evaluate the ability of DMPS and DMSA to extract mercuric ions from placental and fetal tissues following maternal exposure to CH3Hg+. Pregnant Wistar rats were exposed to CH3HgCl, containing [203Hg], on day 11 or day 17 of pregnancy and treated 24 h later with saline, DMPS or DMSA. Maternal organs, fetuses, and placentas were harvested 48 h after exposure to CH3HgCl. The disposition of mercuric ions in maternal organs and tissues was similar to that reported previously by our laboratory. The disposition of mercuric ions in placentas and fetuses appeared to be dependent upon the gestational age of the fetus. The fetal and placental burden of mercury increased as fetal age increased and was reduced by DMPS and DMSA, with DMPS being more effective. The disposition of mercury was examined in liver, total renal mass, and brain of fetuses harvested on gestational day 19. On a per gram tissue basis, the greatest amount of mercury was detected in the total renal mass of the fetus, followed by brain and liver. DMPS and DMSA reduced the burden of mercury in liver and brain while only DMPS was effective in the total renal mass. The results of the current study are the first to show that DMPS and DMSA are capable of extracting mercuric ions, not only from maternal tissues, but also from placental and fetal tissues following maternal exposure to CH3Hg+.
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发表时间：
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影响因子：
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发表时间：
2008-01-01
影响因子：
3.5
作者：
Bridges, Christy C.;Joshee, Lucy;Zalups, Rudolfs K.
通讯作者：
Zalups, Rudolfs K.