Isolated mesopic rod and cone electroretinograms realized with a four-primary method.
Isolated mesopic rod and cone electroretinograms realized with a four-primary method.
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DOI：
10.1007/s10633-011-9279-9
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发表时间：
2011-08
影响因子：
1.4
通讯作者：
Grassi, Michael A.
中科院分区：
文献类型：
--
作者：
Cao, Dingcai;Pokorny, Joel;Grassi, Michael A.
本研究的目的是评估在单一的中间视觉适应水平下测量视杆细胞和视锥细胞视网膜电图（ERG）的可行性。为了实现这一点，使用可商购的ERG系统（Diagnosys ColorDome）实施四初级光刺激器以产生三种类型的刺激，所述三种类型的刺激在时间上单独调制视杆、单独调制视锥以及同时调制视杆和视锥。对于每种刺激类型，ERG被记录为时间频率（2，4，8，或16 Hz）和中间光水平（0.02，0.16，或1.26 cd/m2）在正常观察者和视网膜色素变性（RP）或锥杆变性患者的函数。正常人ERG波形呈明显的周期性变化，与正弦刺激相对应。在所有的光水平，杆响应总是高于锥响应的时间频率在2和8赫兹之间，这表明杆占主导地位的反应。视锥细胞的反应是最小的，在最低的光水平和增加的光水平的增加。对组合刺激的响应幅度介于孤立的视锥细胞和孤立的视杆细胞刺激之间。良好的感受器隔离得到以下结果的证实：（1）患者的视杆细胞ERG极低或无，但可记录到视锥细胞ERG;（2）正常观察者的四原色方法获得的ERG振幅与ISCEV标准临床方案获得的ERG振幅之间高度相关。
The purpose of this study was to evaluate the feasibility of measuring rod and cone electroretinograms (ERGs) at a single mesopic adaptation level. To accomplish this, a four-primary photostimulator was implemented using a commercially available ERG system (Diagnosys ColorDome) to generate three types of stimuli that temporally modulated rods alone, cones alone, and rods and cones simultaneously. For each stimulus type, ERGs were recorded as a function of temporal frequency (2, 4, 8, or 16 Hz) and mesopic light levels (0.02, 0.16, or 1.26 cd/m2) in normal observers and patients with retinitis pigmentosa (RP) or cone–rod degeneration. The normal observers ERG waveforms showed a clear periodic pattern, mirroring the sinusoidal stimuli. At all light levels, rod responses were always higher than cone responses for temporal frequencies between 2 and 8 Hz, suggesting that rods dominated the responses. Cone responses were minimal at the lowest light level and increased with increases in light level. The amplitude of the response to the combined stimuli was intermediate between that of the isolated cone and the isolated rod stimuli for all light levels. Good receptoral isolation was confirmed by the results showing (1) minimal or no rod ERGs but recordable cone ERGs in the patients and (2) high correlation between the ERG amplitudes obtained from the four-primary method and those from the ISCEV standard clinical protocol in normal observers.
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