Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test.
Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test.
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重组小反刍兽疫病毒的拯救：表达 GFP 的病毒的创建及其在快速病毒中和试验中的应用
DOI：
10.1186/1297-9716-43-48
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发表时间：
2012-06-02
影响因子：
4.4
通讯作者：
Bu Z
中科院分区：
文献类型：
--
作者：
Hu Q;Chen W;Huang K;Baron MD;Bu Z
小反刍兽疫病毒（PPRV）在山羊和绵羊中引起高死亡率，并且在过去15年中，该疾病的地理分布大大增加。它对一些最贫穷的发展中国家的严重社会经济问题负有责任。制造重组PPRV的能力将为研究病毒的生物学和疾病的病理学以及开发新的疫苗和诊断方法提供有用的工具。在这里，我们报告的第一个成功的救援重组PPRV从全长cDNA克隆的病毒基因组。通过使用RNA聚合酶II启动子驱动全长病毒反基因组的转录，实现了PPRV的成功回收。我们已经使用该技术构建了表达示踪蛋白（绿色荧光蛋白，GFP）的病毒。重组病毒的复制以及亲本病毒，并可以稳定表达GFP在至少10代。新建立的PPRV反向遗传学系统为以PPRV为载体构建疫苗提供了一种新的方法，也将证明对病毒生物学的基础研究有价值。我们发现，我们的重组病毒可以更快速和更高的通量评估PPRV中和抗体滴度通过病毒中和试验（VNT）相比，传统的方法。
Peste des petits ruminants virus (PPRV) causes high mortality in goats and sheep and the disease has shown a greatly increased geographic distribution over the last 15 years. It is responsible for serious socioeconomic problems in some of the poorest developing countries. The ability to create recombinant PPRV would provide a useful tool for investigating the biology of the virus and the pathology of disease, as well as for developing new vaccines and diagnostic methods. Here we report the first successful rescue of recombinant PPRV from a full-length cDNA clone of the virus genome. Successful recovery of PPRV was achieved by using a RNA polymerase II promoter to drive transcription of the full-length virus antigenome. We have used this technique to construct a virus expressing a tracer protein (green fluorescent protein, GFP). The recombinant virus replicated as well as the parental virus and could stably express GFP during at least 10 passages. The newly established reverse genetics system for PPRV provides a novel method for constructing a vaccine using PPRV as a vector, and will also prove valuable for fundamental research on the biology of the virus. We found that our recombinant virus allowed more rapid and higher throughput assessment of PPRV neutralization antibody titer via the virus neutralization test (VNT) compared with the traditional method.
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影响因子：
5.5
作者：
Rimmelzwaan, Guus F.;Verburgh, R. Joyce;Osterhaus, Albert D. M. E.
通讯作者：
Osterhaus, Albert D. M. E.
影响因子：
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Wang Z;Bao J;Wu X;Liu Y;Li L;Liu C;Suo L;Xie Z;Zhao W;Zhang W;Yang N;Li J;Wang S;Wang J
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作者：
Bailey, Dalan;Chard, Louisa S.;Banyard, Ashley C.
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影响因子：
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作者：
Baron, MD;Foster-Cuevas, M;Barrett, T
通讯作者：
Barrett, T
影响因子：
5.5
作者：
Chen, Weiye;Hu, Sen;Bu, Zhigao
通讯作者：
Bu, Zhigao