Inherited nuclear pore substructures template post-mitotic pore assembly.
Inherited nuclear pore substructures template post-mitotic pore assembly.
复制标题
DOI：
10.1016/j.devcel.2021.05.015
复制
发表时间：
2021-06-21
影响因子：
11.8
通讯作者：
Kirchhausen T
中科院分区：
文献类型：
--
作者：
Chou YY;Upadhyayula S;Houser J;He K;Skillern W;Scanavachi G;Dang S;Sanyal A;Ohashi KG;Di Caprio G;Kreutzberger AJB;Vadakkan TJ;Kirchhausen T
在有丝分裂晚期，核膜组装包括数千个完整的核孔复合体（NPC）的快速形成。NPC组分（核孔蛋白或“NUPs”）的这种有效使用对于确保每个子细胞中的即时核质通讯是必不可少的。我们发现，八聚体的外部和内部核孔环保持完整的有丝分裂内质网（ER）后，NPC解体在前期。这些“遗传的”DNA然后在有丝分裂后孔形成期间并入NPC中。我们进一步表明，通过多轮细胞分裂和伴随的NPC有丝分裂解体和有丝分裂后装配轮，稳定的细胞分裂持续存在。然后，在间期期间从新合成的NUPs从头形成NPC将具有不同的起始机制。我们假设，一个尚未确定的修改标记和“永生化”的一个或多个组件的特定八聚体的外环和内环亚复合物，然后模板后有丝分裂NPC大会在随后的细胞周期。Chou等人的研究表明，细胞从其曾曾曾曾曾曾祖母那里继承了内环和外环核孔结构。这些“永生”结构，在有丝分裂期间保留在高度有孔ER片中，作为有丝分裂后核孔复合物（NPC）组装的模板，因为ER片在两个子细胞中的每一个中重新涂覆染色质团。
Nuclear envelope assembly during late mitosis includes rapid formation of several thousand complete nuclear pore complexes (NPCs). This efficient use of NPC components (nucleoporins or “NUPs”) is essential for ensuring immediate nucleocytoplasmic communication in each daughter cell. We show that octameric subassemblies of outer and inner nuclear pore rings remain intact in the mitotic endoplasmic reticulum (ER) after NPC disassembly during prophase. These “inherited” subassemblies then incorporate into NPCs during post-mitotic pore formation. We further show that the stable subassemblies persist through multiple rounds of cell division and the accompanying rounds of NPC mitotic disassembly and post-mitotic assembly. De novo formation of NPCs from newly synthesized NUPs during interphase will then have a distinct initiation mechanism. We postulate that a yet-to-be identified modification marks and “immortalizes” one or more components of the specific octameric outer- and inner-ring subcomplexes that then template post-mitotic NPC assembly during subsequent cell cycles. Chou et al. shows that cells inherit inner and outer ring nuclear pore subassemblies from their great-great-great-great-great-great-great-great grandmothers. These “immortal” structures, retained during mitosis in highly fenestrated ER sheets, template post-mitotic nuclear pore complex (NPC) assembly as ER sheets recoat the chromatin masses in each of the two daughter cells.
登录
查看更多内容
影响因子：
8.8
作者：
Forozan, F;Veldman, R;Ammerman, CA;Parsa, NZ;Kallioniemi, A;Kallioniemi, OP;Ethier, SP
通讯作者：
Ethier, SP
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
5.4
作者：
Cureton, David K.;Harbison, Carole E.;Kirchhausen, Tom
通讯作者：
Kirchhausen, Tom
影响因子：
48
作者：
Grimm JB;Muthusamy AK;Liang Y;Brown TA;Lemon WC;Patel R;Lu R;Macklin JJ;Keller PJ;Ji N;Lavis LD
通讯作者：
Lavis LD
涂有网状蛋白涂层坑的前五秒钟。
影响因子：
64.5
作者：
Cocucci E;Aguet F;Boulant S;Kirchhausen T
通讯作者：
Kirchhausen T