Three-Dimensional Quantification of Collagen Microstructure During Tensile Mechanical Loading of Skin.
Three-Dimensional Quantification of Collagen Microstructure During Tensile Mechanical Loading of Skin.
复制标题
皮肤拉伸力学加载过程中胶原蛋白微观结构的三维定量研究。
DOI：
10.3389/fbioe.2021.642866
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发表时间：
2021
影响因子：
5.7
通讯作者：
Quinn KP
中科院分区：
文献类型：
--
作者：
Woessner AE;Jones JD;Witt NJ;Sander EA;Quinn KP
皮肤是一种异质组织，随着年龄、疾病或损伤，其结构和功能会发生显著变化。了解这些变化如何影响皮肤的机械性能，需要三维（3D）量化的组织微观结构及其运动学。本研究的目的是通过二次谐波发生（SHG）显微镜下的小鼠皮肤拉伸机械负荷，以量化这些结构与功能的关系。在宏观和微观尺度上的组织变形被量化，并且在拉伸的情况下检测到组织体积的大幅减少和大的泊松比，这表明皮肤与历史上用于解释其行为的超弹性材料模型有很大不同。此外，测量的应变的相对量在长度尺度之间没有显著变化，表明胶原纤维网络均匀分布施加的应变。未变形的胶原纤维组织和体积分数的分析揭示了这两个指标的长度尺度依赖性。SHG体积的3D分析还表明，胶原纤维排列在拉伸方向上增加，但纤维体积分数没有改变。有趣的是，发现3D纤维运动学与组织变形具有非仿射关系，并且微尺度纤维网络的仿射变换高估了纤维重新排列的量。这一结果与其他结果沿着，突出了在开发皮肤机械功能的多尺度模型时，精确、尺度匹配的3D实验测量的重要性。
Skin is a heterogeneous tissue that can undergo substantial structural and functional changes with age, disease, or following injury. Understanding how these changes impact the mechanical properties of skin requires three-dimensional (3D) quantification of the tissue microstructure and its kinematics. The goal of this study was to quantify these structure-function relationships via second harmonic generation (SHG) microscopy of mouse skin under tensile mechanical loading. Tissue deformation at the macro- and micro-scale was quantified, and a substantial decrease in tissue volume and a large Poisson’s ratio was detected with stretch, indicating the skin differs substantially from the hyperelastic material models historically used to explain its behavior. Additionally, the relative amount of measured strain did not significantly change between length scales, suggesting that the collagen fiber network is uniformly distributing applied strains. Analysis of undeformed collagen fiber organization and volume fraction revealed a length scale dependency for both metrics. 3D analysis of SHG volumes also showed that collagen fiber alignment increased in the direction of stretch, but fiber volume fraction did not change. Interestingly, 3D fiber kinematics was found to have a non-affine relationship with tissue deformation, and an affine transformation of the micro-scale fiber network overestimates the amount of fiber realignment. This result, along with the other outcomes, highlights the importance of accurate, scale-matched 3D experimental measurements when developing multi-scale models of skin mechanical function.
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影响因子：
2.4
作者：
LANIR, Y
通讯作者：
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影响因子：
11
作者：
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影响因子：
4.6
作者：
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通讯作者：
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