Putative calcium channels CchA and MidA play the important roles in conidiation, hyphal polarity and cell wall components in Aspergillus nidulans.
Putative calcium channels CchA and MidA play the important roles in conidiation, hyphal polarity and cell wall components in Aspergillus nidulans.
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假定的钙通道 CchA 和 MidA 在构巢曲霉的分生孢子形成、菌丝极性和细胞壁成分中发挥重要作用
DOI：
10.1371/journal.pone.0046564
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lu L
中科院分区：
文献类型：
--
作者：
Wang S;Cao J;Liu X;Hu H;Shi J;Zhang S;Keller NP;Lu L
虽然高亲和力的Ca2+通道Cch1及其亚基Mid1已经在酵母和一些丝状真菌中进行了研究和评估，但对其同源物在曲霉中的功能知之甚少。在这里，我们用条件缺失突变体和零缺失突变体对细粒曲霉的酵母菌同源物CchA和MidA进行了功能表征。CchA和MidA不仅具有与酵母中Cch1和Mid1一致的快速生长的功能效益，而且在低钙环境下调节分生、菌丝极性和细胞壁成分等方面具有独特而复杂的作用。CchA或MidA缺陷导致分生孢子数量急剧减少，伴生芽胞异常，在较高的接种密度下，分生孢子不发育。最有趣的是，突变体中的这些分生缺陷可以通过钙调磷酸酶依赖的细胞外Ca2+或钙调磷酸酶不依赖的渗透胁迫来明显地修复。此外，表型缺陷不会因双缺失的存在而加剧，再加上Y2H测定，表明CchA和MidA可能形成一个复合物，共同发挥作用。我们的研究结果表明，高亲和力的Ca2+通道可能是一种可行的、完全未被探索的途径，可以减少曲霉的分生作用。
Although the high affinity Ca2+ channel, Cch1, and its subunit Mid1 have been investigated and evaluated in yeast and some of filamentous fungi, little is known about the function of their homologs in the Aspergilli. Here, we have functionally characterized the yeast homologs, CchA and MidA, in Aspergillus nidulans using conditional and null deletion mutants. CchA and MidA not only have functional benefits of fast growth, which is consistent with Cch1 and Mid1 in yeast, but also have unique and complex roles in regulating conidiation, hyphal polarity and cell wall components in low-calcium environments. The defect of CchA or MidA resulted in a sharp reduction in the number of conidiospores, accompanied by abnormal metulae, and undeveloped-phialides at a higher density of inoculum. Most interestingly, these conidiation defects in mutants can, remarkably, be rescued either by extra-cellular Ca2+ in a calcineurin-dependent way or by osmotic stress in a calcineurin-independent way. Moreover, the fact that the phenotypic defects are not exacerbated by the presence of the double deletion, together with the Y2H assay, indicates that CchA and MidA may form a complex to function together. Our findings suggest that the high-affinity Ca2+ channel may represent a viable and completely unexplored avenue to reduce conidiation in the Aspergilli.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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通讯作者：
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通讯作者：
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