SU-8 photoresist as material of optical passive components integrated with analytical microsystems for real-time polymerase chain reaction
SU-8 photoresist as material of optical passive components integrated with analytical microsystems for real-time polymerase chain reaction
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SU-8光刻胶作为与实时聚合酶链反应分析微系统集成的光学无源元件材料
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
J. Dziuban
中科院分区：
文献类型：
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作者：
R. Walczak;P. Śniadek;J. Dziuban
分析微系统的快速发展，通常也被称为芯片上的实验室，用于对纳米和皮体积的样品进行各种化学和生物化学分析，已经观察了近二十年。分析微系统在医疗和兽医实践中的成功应用与许多因素有关，但其中之一是一次性微系统的低价格-通常以芯片的形式。芯片由廉价材料制成，例如聚合物。这种材料之一是负性光致抗蚀剂SU-8。它的特点是生物相容性和容易制造的各种三维流体微结构的可能性。此外，SU-8对可见光透明。它使SU-8成为芯片实验室的有吸引力的材料，专用于通过实时聚合酶链反应（PCR）进行遗传物质分析。在本文中，我们提出了类似PCR的温度分布的透射光谱的影响的调查结果。研究了SU-8分子在不同激光照射下的自发荧光效应对灵敏荧光读出的影响。所获得的结果表明，SU-8可以成功地应用为芯片实验室材料，但是，由于SU-8的高自发荧光，当需要高灵敏度荧光检测时，红线荧光染料是优选的。
Rapid development of analytical microsystems, also often called as labs-on-a-chip and used to perform various chemical and biochemical analysis with nano- and pico-volumes of the sample, has been observed for almost two decades. Successful application of analytical microsystems in medical and veterinary practices is relevant to many factors but one of them is low price of the disposable microsystem – often in the form of a chip. The chips are made of cheap materials, for example, polymers. One of such material is a negative photoresist SU-8. It is characterized by biocompatibility and possibility of easy fabrication of various three-dimensional fluidic microstructures. Moreover, SU-8 is transparent for visible light. It makes SU-8 attractive material for labs-on-a-chip dedicated for genetic material analysis by real-time polymerase chain reaction (PCR). In this paper, we present the results of investigations of the influence of PCR-like temperature profiling on the transmittance spectra. The autofluorescence effect of SU-8 illuminated with various lasers has also been investigated as one of the factors limiting sensitive fluorescence readout. The results obtained showed that SU-8 can be successfully applied as labs-on-a-chip material but, due to high SU-8 autofluorescence, red-line fluorochromes are preferred when high-sensitivity fluorescence detection is required.
影响因子：
14
作者：
Voskerician, G;Shive, MS;Langer, R
通讯作者：
Langer, R