Selective activation of a discrete family of endogenous proviral elements in normal BALB/c lymphocytes
Selective activation of a discrete family of endogenous proviral elements in normal BALB/c lymphocytes
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正常 BALB/c 淋巴细胞中内源性前病毒元件离散家族的选择性激活
DOI：
--
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发表时间：
1992
影响因子：
5.3
通讯作者：
E. Kuff
中科院分区：
文献类型：
--
作者：
J. Mietz;J. Fewell;E. Kuff
胞内a粒子（IAP）原病毒元件丰富且广泛分布于小鼠基因组中。在正常小鼠组织中检测到iap相关转录物，其表达受遗传控制。在这项研究中，我们试图确定在BALB/c胸腺和脂多糖刺激的B细胞中IAP表达是由于IAP元件的选择性激活还是不加选择地激活。从每个来源制备cDNA文库。从两个文库中共分离到86个IAP cDNA克隆，其中37个克隆在IAP基因组的0.7- 1.0 kb区域（包括3'长末端重复序列（LTR））上进行了测序。三个高度相关的元件家族被发现在两种细胞类型中表达。所有相关元件都有一个独特的U3调控区。根据LTR R区域内的序列差异，区分出13个IAP原病毒元件。基因组DNA与元件特异性寡核苷酸探针杂交证实，在淋巴细胞特异性元件家族中存在有限数量的原病毒拷贝。大多数这些拷贝在淋巴细胞DNA中被甲基化，但至少有七个在它们的5' ltr中被低甲基化。本研究表明，正常小鼠淋巴细胞中IAP元素的激活具有高度选择性。激活可能与原病毒LTR的序列特异性和甲基化状态有关。
Intracisternal A-particle (IAP) proviral elements are abundant and widely dispersed in the mouse genome. IAP-related transcripts have been detected in normal mouse tissues where expression is under genetic control. In this study, we sought to determine whether IAP expression in BALB/c thymus and lipopolysaccharide-stimulated B cells was due to selective or indiscriminate activation of IAP elements. cDNA libraries were prepared from each source. A total of 86 IAP cDNA clones were isolated from both libraries, and 37 of these were sequenced over a common 0.7- to 1.0-kb region of the IAP genome that included the 3' long terminal repeat (LTR). Three highly related families of elements were found to be expressed in the two cell types examined. All of the related elements had a distinctive U3 regulatory region. Thirteen individual IAP proviral elements were distinguished on the basis of sequence differences within the R region of the LTR. Hybridization of genomic DNA with element-specific oligonucleotide probes confirmed the presence of a restricted number of proviral copies in the lymphocyte-specific family of elements. Most of these copies were found to be methylated in the lymphocyte DNA, but at least seven were hypomethylated in their 5' LTRs. This study shows that activation of IAP elements in normal normal mouse lymphocytes is highly selective. Activation is probably a function of both sequence specificity and methylation status of the proviral LTR.
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移码诱变 DNA 中间体的聚合酶特异性差异。
影响因子：
5.6
作者：
Papanicolaou,C;Ripley,LS
通讯作者：
Ripley,LS
编码来自大鼠-小鼠 T 细胞杂交瘤的 IgE 结合因子的 cDNA 克隆。
DOI：
10.1073/pnas.82.8.2460
发表时间：
1985
影响因子：
11.1
作者：
Martens,CL;Huff,TF;Jardieu,P;Trounstine,ML;Coffman,RL;Ishizaka,K;Moore,KW
通讯作者：
Moore,KW
鼠A型逆转录病毒促进胚胎癌细胞中高水平的基因表达。
DOI：
10.1242/dev.102.1.23
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Morgan,RA;Christy,RJ;Huang,RC
通讯作者：
Huang,RC
小鼠胰腺 B 细胞中的脑池内 A 型颗粒。
DOI：
--
发表时间：
1984
期刊：
The American journal of pathology
影响因子：
--
作者：
Leiter,EH;Kuff,EL
通讯作者：
Kuff,EL