Effects of sulfhydryl and other reagents on the diffusional permeability of dog erythrocytes to small solutes.
Effects of sulfhydryl and other reagents on the diffusional permeability of dog erythrocytes to small solutes.
复制标题
巯基和其他试剂对狗红细胞对小溶质的扩散渗透性的影响。
DOI：
10.1016/0005-2736(83)90081-0
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
F. Chinard
中科院分区：
文献类型：
--
作者：
R. A. Garrick;B. C. Patel;F. Chinard
在37°C下，用线性扩散技术测定了对氯甲基脲苯磺酸（PCMBS）、5，5 ′-二硫代双（2-硝基苯甲酸）（DTNB）、根皮素和硫脲对狗红细胞对氚水和~（14）C标记的亲脂性和亲水性小分子溶质的扩散渗透性的影响。对3 HHO的渗透性被PCMBS显着降低，但不受其他试剂的影响。根皮素和硫脲降低了小的亲水性溶质乙酰胺和尿素的渗透性，但只有乙酰胺的渗透性降低到统计学显着的程度由PCMBS。对亲脂性溶质甲醇、乙醇和安替比林的渗透性不受任何这些试剂的影响。我们解释这些结果表明，小的亲脂性溶质可能通过脂质区移动，小的亲水性溶质可能通过蛋白质相关的红细胞膜中的区域移动和小的亲水性溶质的途径是不同的水。虽然水的途径可能与膜蛋白相关，但它们似乎并不像对人红细胞所建议的那样与带3蛋白特异性相关。通过狗红细胞的扩散水运动通过两种不同的途径发生。
The effects ofp-chloromericuriphenylsulfonic acid (PCMBS), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), phloretin and thiourea on the diffusional permeability of dog erythrocytes to tritiated water and to small14C-labeled lipophilic and hydrophilic solutes were measured at 37°C by means of the linear diffusion technique. Permeability to3HHO was significantly decreased by PCMBS but was not affected by the other reagents. The permeability to the small hydrophilic solutes acetamide and urea was decreased by phloretin and thiourea but only the permeability to acetamide was reduced to a statistically significant extent by PCMBS. The permeability to the lipophilic solutes methanol, ethanol and antipyrine was not affected by any of these agents. We interpret these results as an indication that the small lipophilic solutes probably move through lipid areas, that the small hydrophilic solutes probably move through protein associated areas in the erythrocyte membrane and that pathways for the small hydrophilic solutes are distinct from those for water. While the pathways for water may be associated with membrane protein they do not appear to be associated specifically with band 3 protein as has been suggested for human erythrocytes. Diffusional water movement through the dog erythrocyte occurs by two distinct pathways.
DOI：
10.1016/0005-2736(80)90455-1
发表时间：
1980
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Kutchai,H;Cooper,RA;Forster,RE
通讯作者：
Forster,RE