Ericoid mycorrhizal root fungi and their multicopper oxidases from a temperate forest shrub.
Ericoid mycorrhizal root fungi and their multicopper oxidases from a temperate forest shrub.
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DOI：
10.1002/ece3.67
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发表时间：
2012-01
影响因子：
2.6
通讯作者：
Hendrick, Ronald L.
中科院分区：
文献类型：
--
作者：
Wurzburger, Nina;Higgins, Brian P.;Hendrick, Ronald L.
杜鹃花科菌根真菌（ERM）可能专门从其宿主的凋落物中捕获营养物质，作为调节陆地生态系统营养循环的一种策略。尽管它们具有潜在的意义，但我们对ERM真菌群落的结构及其腐生性的遗传基础知之甚少（例如，编码胞外酶的基因）。大杜鹃是一种典型的ERM林下灌木，影响了阿巴拉契亚山脉南部（北卡罗来纳州，美国）山地阔叶林的养分循环。我们采样了R的ERM根。最大的有机和矿质土壤的视野和确定根真菌的扩增和测序的内部转录间隔区（ITS）核糖体DNA（rDNA）收集的文化和克隆。我们观察到71种真菌分类群的ERM根，包括已知的共生Rhizoscyphus ericae和Oidiodendron maius，推定的共生菌从Helotiales，Chaetocomalales，和Sebacinales，外生菌根共生菌，和saprotrophs。支持的想法，ERM真菌是熟练的腐殖生物，丰富的根真菌是有机比矿质土壤的视野。为了研究参与分解的氧化酶的遗传多样性，我们扩增并测序了ERM子囊菌中编码多铜氧化酶（MCOs）的部分基因。大多数真菌具有多个拷贝的MCO序列与已知的铁氧化酶和漆酶具有很强的相似性。我们的研究结果表明，R.最大关联分类和生态多样性的真菌群落。对根际真菌MCO基因多样性和表达的研究有助于了解根际真菌是如何调控寄主植物与土壤环境之间的养分循环的。
Ericoid mycorrhizal fungi (ERM) may specialize in capturing nutrients from their host's litter as a strategy for regulating nutrient cycles in terrestrial ecosystems. In spite of their potential significance, we know little about the structure of ERM fungal communities and the genetic basis of their saprotrophic traits (e.g., genes encoding extracellular enzymes). Rhododendron maximum is a model ERM understory shrub that influences the nutrient cycles of montane hardwood forests in the southern Appalachians (North Carolina, USA). We sampled ERM roots of R. maximum from organic and mineral soil horizons and identified root fungi by amplifying and sequencing internal transcribed spacer (ITS) ribosomal DNA (rDNA) collected from cultures and clones. We observed 71 fungal taxa on ERM roots, including known symbionts Rhizoscyphus ericae and Oidiodendron maius, putative symbionts from the Helotiales, Chaetothyriales, and Sebacinales, ectomycorrhizal symbionts, and saprotrophs. Supporting the idea that ERM fungi are adept saprotrophs, richness of root-fungi was greater in organic than in mineral soil horizons. To study the genetic diversity of oxidative enzymes that contribute to decomposition, we amplified and sequenced a portion of genes encoding multicopper oxidases (MCOs) from ERM ascomycetes. Most fungi possessed multiple copies of MCO sequences with strong similarities to known ferroxidases and laccases. Our findings indicate that R. maximum associates with a taxonomically and ecologically diverse fungal community. The study of MCO gene diversity and expression may be useful for understanding how ERM root fungi regulate the cycling of nutrients between the host plant and the soil environment.
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影响因子：
4.2
作者：
Kellner, Harald;Luis, Patricia;Buscot, Francois
通讯作者：
Buscot, Francois
影响因子：
4.9
作者：
GARDES, M;BRUNS, TD
通讯作者：
BRUNS, TD
影响因子：
9.7
作者：
Bending, GD;Read, DJ
通讯作者：
Read, DJ
影响因子：
16.5
作者：
Crous, Pedro W;Verkley, Gerard J M;Groenewald, Johannes Z
通讯作者：
Groenewald, Johannes Z
影响因子：
--
作者：
Bending, GD;Read, DJ
通讯作者：
Read, DJ