Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC-MS/MS.
Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC-MS/MS.
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作者：
Kjellin LL;Dorsey G;Rosenthal PJ;Aweeka F;Huang L
儿科血浆中哌喹（PQ）的测定需要一种小样品体积的方法。我们报告了一种灵敏的LC-MS/MS方法，用于仅使用25 µI人血浆定量PQ。使用氘代内标物（PQ-d 6）、分析型PFP色谱柱、APCI+作为离子源和MRM（PQ为535/288，IS为541/294）进行检测，该方法的线性校准范围为1.5-250 ng/ml，每个样品的运行时间为3.0 min。该方法适用于儿童血浆样品。开发的LC-MS/MS方法适用于通过毛细管采集小体积血浆样品的儿科研究。一个限制是PFP色谱柱的性能在不同品牌之间存在差异。
Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume. We report a sensitive LC–MS/MS method for quantitation of PQ with only 25 µI human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI+ as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5–250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children. The developed LC–MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.
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