Imaging nanometre-scale structure in cells using in situ aberration correction.
Imaging nanometre-scale structure in cells using in situ aberration correction.
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DOI：
10.1111/j.1365-2818.2012.03654.x
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发表时间：
2012-10
影响因子：
2
通讯作者：
Straight AF
中科院分区：
文献类型：
--
作者：
Fuller CJ;Straight AF
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Accurate distance measurements of cellular structures on a length scale relevant to single macromolecules or macro-molecular complexes present a major challenge for biological microscopy. In addition to the inherent challenges of overcoming the limits imposed by the diffraction of light, cells themselves are a complex and poorly understood optical environment. We present an extension of the high-resolution colocalization method to measure three dimensional distances between diffraction-limited objects using standard widefield fluorescence microscopy. We use this method to demonstrate that in three dimensions, cells intrinsically introduce a large and variable amount of chromatic aberration into optical measurements. We present a means of correcting this aberration in situ (termed “Colocalization and In-situ Correction of Aberration for Distance Analysis,” CICADA) by exploiting the fact that there is a linear relationship between the degree of aberration between different wavelengths. By labeling a cellular structure with redundantly multicolor labeled antibodies, we can create an intracellular fiducial marker for correcting the aberrations between two different wavelengths in the same cells. Our observations demonstrate that with suitable corrections, nanometer scale three-dimensional distance measurements can be used to probe the substructure of macromolecular complexes within cells.
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影响因子：
16.2
作者：
Dani, Adish;Huang, Bo;Bergan, Joseph;Dulac, Catherine;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
2
作者：
Hell, SW;Schrader, M;VanderVoort, HTM
通讯作者：
VanderVoort, HTM
影响因子：
10.8
作者：
Hoyer, Patrick;Staudt, Thorsten;Hell, Stefan W.
通讯作者：
Hell, Stefan W.
DOI：
10.1016/j.cub.2009.02.056
发表时间：
2009-04-28
期刊：
Current biology : CB
影响因子：
--
作者：
Joglekar AP;Bloom K;Salmon ED
通讯作者：
Salmon ED
DOI：
10.1073/pnas.071275698
发表时间：
2001-03-27
影响因子：
11.1
作者：
Kam, Z;Hanser, B;Sedat, JW
通讯作者：
Sedat, JW