DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.
DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.
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DOI：
10.1111/mmi.13920
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发表时间：
2018-04
影响因子：
3.6
通讯作者：
Merrikh H
中科院分区：
文献类型：
--
作者：
Samadpour AN;Merrikh H
在细菌中，DNA复制的起始需要DnaA蛋白。DnaA协会和活动的复制起点，oriC的调节，是复制起始控制的主要机制。已知对复制通常重要的一个关键特征是DNA拓扑结构。虽然有一些建议，拓扑结构可能会影响复制起始，是否这种机制调节DNA介导的复制起始尚不清楚。我们发现，必要的拓扑异构酶，DNA旋转酶，是所需的DnaA的oriC的适当的结合，以及在枯草芽孢杆菌的起始频率的控制。此外，我们发现，在启动的促旋酶的调节活性是特异性的DnaA和oriC。从DNA非依赖性起点oriN启动复制的细胞在很大程度上对新生霉素的促旋酶抑制具有抗性，即使在oriC细胞中危及存活的浓度高达四个数量级。此外，促旋酶的抑制不影响oriN细胞中的起始频率。此外，DnaA调节因子YabA的缺失或过表达显著调节对促旋酶抑制的敏感性，但仅在oriC细胞中，而不是oriN细胞中。我们建议，促旋酶是一个负调节DnaA依赖的复制起始oriC，这种调节机制是细胞生存所必需的。DNA促旋酶通过抑制复制起点oriC处的DnaA结合和活性来控制复制起始。促旋酶的抑制增加复制起始频率和DnaA与oriC的结合，并且如果复制起始于oriC则对细胞存活有害。我们提出了一个模型，在orisome装配过程中的早期阶段，通过促旋酶调节DNA拓扑结构来调节复制起始。
In bacteria, initiation of DNA replication requires the DnaA protein. Regulation of DnaA association and activity at the origin of replication, oriC, is the predominant mechanism of replication initiation control. One key feature known to be generally important for replication is DNA topology. Although there have been some suggestions that topology may impact replication initiation, whether this mechanism regulates DnaA-mediated replication initiation is unclear. We found that the essential topoisomerase, DNA gyrase, is required for both proper binding of DnaA to oriC as well as control of initiation frequency in Bacillus subtilis. Furthermore, we found that the regulatory activity of gyrase in initiation is specific to DnaA and oriC. Cells initiating replication from a DnaA-independent origin, oriN, are largely resistant to gyrase inhibition by novobiocin, even at concentrations that compromise survival by up to four orders of magnitude in oriC cells. Furthermore, inhibition of gyrase does not impact initiation frequency in oriN cells. Additionally, deletion or overexpression of the DnaA regulator, YabA, significantly modulates sensitivity to gyrase inhibition, but only in oriC and not oriN cells. We propose that gyrase is a negative regulator of DnaA-dependent replication initiation from oriC, and that this regulatory mechanism is required for cell survival. DNA gyrase controls replication initiation by inhibiting DnaA binding and activity at the origin of replication, oriC. Inhibition of gyrase increases replication initiation frequency and DnaA association with oriC, and is harmful to cell survival if replication initiates from oriC. We propose a model where modulation of DNA topology by gyrase regulates replication initiation at an early step during orisome assembly.
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DOI：
10.1073/pnas.97.17.9419
发表时间：
2000-08-15
影响因子：
11.1
作者：
Khodursky, AB;Peter, BJ;Cozzarelli, NR
通讯作者：
Cozzarelli, NR
影响因子：
11.4
作者：
ASAI, T;TAKANAMI, M;IMAI, M
通讯作者：
IMAI, M
影响因子：
3.2
作者：
Goranov, Alexi I.;Kuester-Schoeck, Elke;Grossman, Alan D.
通讯作者：
Grossman, Alan D.
影响因子：
3.6
作者：
Kaur, Gulpreet;Vora, Mansi P.;Leonard, Alan C.
通讯作者：
Leonard, Alan C.
DOI：
10.1073/pnas.80.19.5817
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FULLER, RS;KORNBERG, A
通讯作者：
KORNBERG, A