Viability variation of T-cells under ultrasound exposure according to adhesion condition with bubbles
Viability variation of T-cells under ultrasound exposure according to adhesion condition with bubbles
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超声波照射下 T 细胞的活力随气泡粘附情况的变化
DOI：
10.1007/s10396-022-01277-5
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发表时间：
2023
影响因子：
1.8
通讯作者：
Masuda Kohji
中科院分区：
文献类型：
--
作者：
Kajita Naoya;Ito Yoshiki;Watanabe Shunya;Miyamoto Yoshitaka;Omata Daiki;Maruyama Kazuo;Suzuki Ryo;Masuda Kohji
虽然细胞免疫疗法有望成为一种新的癌症治疗方法，但其在实体瘤中的治疗效果有限，因为大多数细胞返回血流而不是粘附到靶部位。因此，我们的动机是开发一种技术，以集中在血流中的细胞，使用主动控制的气泡包围的细胞在超声照射下考虑两个方面的细胞的可控性和violence.MethodsWe制备了一个脂泡共轭配体粘附到T细胞的表面。首先，我们通过连续超声照射将细胞保持在人造血管壁上来评估细胞可控性。接下来，我们研究了在具有不同气泡浓度的悬浮液中超声照射下的细胞活力。结果我们估计了当粘附到细胞表面饱和时的气泡浓度。然后，我们用不同的超声波暴露条件和气泡浓度来评估细胞活力。2然而，证实了在适当控制细胞的条件下会发生细胞损伤。因此，我们将细胞暴露于突发波以降低施加的超声强度。因此，细胞活力的显着增加被证实是成反比的占空比。ConclusionTo保留细胞在血管壁上，确定适当的超声条件，包括声压和波形是重要的，以保持细胞活力。
PurposeAlthough cellular immunotherapy is expected as a new cancer treatment, its therapeutic efficiency is limited in solid tumors, because most cells return to the bloodstream rather than adhere to the target site. Therefore, we are motivated to develop a technique to concentrate the cells in the blood flow using active control of bubble-surrounded cells under ultrasound exposure considering both aspects of cell controllability and viability.MethodsWe prepared a lipid bubble conjugating ligand to adhere to the surface of the T-cells. First, we evaluated the cell controllability by retaining the cells on a wall of an artificial blood vessel through continuous ultrasound exposure. Next, we investigated the cell viability under ultrasound exposure in a suspension with various bubble concentrations.ResultsWe estimated the concentration of bubbles when the adhesion to the cell surface was saturated. Then, we evaluated the cell viability with various conditions of ultrasound exposure and bubble concentrations. However, it was confirmed that cell damage occurred under conditions that achieved proper control of the cells. Therefore, we exposed the cells to burst waves to reduce the applied ultrasound intensity. Consequently, the significant increase in cell viability was confirmed to be inversely proportional to the duty ratio.ConclusionTo retain cells on a vessel wall, determining the appropriate ultrasound condition including sound pressure and waveform is important to maintain cell viability.
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影响因子：
6
作者：
Yokoe I;Omata D;Unga J;Suzuki R;Maruyama K;Okamoto Y;Osaki T
通讯作者：
Osaki T
通过形成聚集体主动控制血流中微泡的实验研究
DOI：
10.1109/ultsym.2011.0503
发表时间：
2011
期刊：
2011 IEEE International Ultrasonics Symposium
影响因子：
--
作者：
K. Masuda;R. Koda;N. Watarai;N. Shigehara;T. Ito;T. Kakimoto;Y. Miyamoto;S. Enosawa;T. Chiba
通讯作者：
T. Chiba
影响因子：
3.4
作者：
Kudo, Nobuki;Okada, Kengo;Yamamoto, Katsuyuki
通讯作者：
Yamamoto, Katsuyuki
不同超声条件下分叉流中气泡脂质体主动控制的评价
影响因子：
1
作者：
Ren Koda;Jun Koido;Naoto Hosaka;Shinya Onogi;Takashi Mochizuki;Kohji Masuda;Ryo Suzuki;Kazuo Maruyama
通讯作者：
Kazuo Maruyama
StemBell 疗法可稳定心肌梗塞后的动脉粥样硬化斑块。
影响因子：
4.5
作者：
L. Woudstra;E. Meinster;Laura VAN Haren;Amber M. Kay;M. Koopman;J. Belien;M. Morrison;A. V. van Rossum;M. Helder;L. Juffermans;H. Niessen;P. Krijnen
通讯作者：
P. Krijnen