Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection
Identification of viruses infecting sweet potato in southern China by small RNA deep sequencing and PCR detection
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小RNA深度测序和PCR检测鉴定华南甘薯感染病毒
DOI：
10.1007/s10327-018-0832-1
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发表时间：
2018-11
影响因子：
1.2
通讯作者：
Zhang Tong
中科院分区：
文献类型：
--
作者：
Ma Siqi;Zheng Qiufeng;Ye Jiajie;Feng Wendi;Zhou Guohui;Zhang Tong
甘薯是世界上最重要的粮食作物之一，病毒病可导致甘薯严重的产量损失和品质下降。为追踪甘薯病毒病的田间流行情况，从我国南方10个甘薯产区采集了219份甘薯病毒病样品，采用小分子RNA深度测序和PCR/RT-PCR技术进行了分析。结果表明，78.54%的样品有病毒感染，共鉴定出11种病毒。甘薯G病毒和甘薯羽状斑驳病毒的发病率最高，在40%以上的样品中检测到。甘薯病毒混合侵染在我国南方地区较为普遍，48%的样品同时被两种或两种以上的病毒侵染。这些结果表明，甘薯病毒在中国南方普遍存在，并强调了准确诊断的重要性，以检测和生产无病毒植物的要求。
Sweet potato is one of the most important food crops worldwide, and viral diseases can cause very severe yield loss and quality decline of this crop. To track the prevalence of viral diseases of sweet potato in the field, 219 samples were collected from 10 locations in southern China and analysed by small RNA deep sequencing and PCR/RT-PCR. The results showed that 78.54% of the samples were infected with viruses, and 11 virus species were identified. Sweet potato virus G and sweet potato feathery mottle virus had the highest incidence and were detected in more than 40% of the samples that were tested. Coinfection of sweet potato viruses was common in southern China, and 48% of the samples were simultaneously infected by two or more virus species. These results indicate that sweet potato viruses are prevalent in southern China and highlight the importance of accurate diagnostics for their detection and the requirement for the production of virus-free plants.
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