Maternal antibiotic treatment during pregnancy attenuates the transport and absorption of maternal antibody IgG through TLR4 and TLR2 receptor.
Maternal antibiotic treatment during pregnancy attenuates the transport and absorption of maternal antibody IgG through TLR4 and TLR2 receptor.
复制标题
妊娠期间母体抗生素治疗会减弱母体抗体 IgG 通过 TLR4 和 TLR2 受体的转运和吸收
DOI：
10.3389/fmicb.2023.1109273
复制
发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
母体抗体IgG是初乳中的主要抗体，在新生儿保护中起着重要作用。共生菌群与宿主抗体库密切相关。然而，关于母体肠道菌群如何影响母体抗体IgG转移的报道很少。在本研究中，我们研究了改变肠道菌群（妊娠期间使用抗生素治疗）对母体IgG运输和子代吸收的影响，并探讨了其潜在机制。结果表明，妊娠期抗生素治疗显著降低了母胎盲肠微生物丰富度（Chao1和Obesrved species）和多样性（Shannon和Simpson）。血浆代谢组富集过程中胆汁酸分泌通路发生显著变化，微生物次级代谢物脱氧胆酸浓度降低。流式细胞术分析表明，抗生素治疗可促进大鼠肠固有层中B细胞数量的增加，使T、DC和M1细胞数量减少。令人惊讶的是，抗生素处理后的母猪血清IgG水平显著升高，而初乳中IgG含量降低。此外，妊娠抗生素治疗降低了母鸭乳腺及新生儿十二指肠和空肠中FcRn、TLR4和TLR2的表达。此外，TLR4−/−和TLR2−/−敲除小鼠在母鼠乳房和新生儿十二指肠和空肠中的FcRn表达降低。上述结果提示母体肠道细菌可能通过调节母鼠乳腺TLR4和TLR2影响母体IgG的转移。
Maternal antibody IgG, the main antibody in colostrum, plays an important role in neonates protection. Commensal microbiota is closely related to host antibody repertoire. However, there are few reports on how maternal gut microbiota affects maternal antibody IgG transfer. In the present study, we investigated the effects of altering the gut microbiota (treated with antibiotics during pregnancy) on maternal IgG transportation and offspring absorption and explored its underlying mechanisms. Results showed that antibiotic treatment during pregnancy significantly decreased maternal cecal microbial richness (Chao1 and Obesrved species) and diversity (Shannon and Simpson). Plasma metabolome enriched significant changes in the process of bile acid secretion pathway, and the concentration of deoxycholic acid, a secondary metabolite of microorganisms was lowered. Flow cytometry analysis indicated that antibiotic treatment promoted the number of B cells and abated the number of T, DC and M1 cells in intestinal lamina propria of dams. Surprisingly, the serum IgG level in antibiotic treated dams was significantly increased, while IgG contents in colostrum was decreased. Moreover, pregnancy antibiotic treatment in dams was reduced the expression of FcRn, TLR4 and TLR2 in breast of dams and in duodenum and jejunum of neonates. Furthermore, TLR4−/− and TLR2−/− knock-out mice showed a lower FcRn expression in breast of dams and in duodenum and jejunum of neonates. These findings suggest that maternal intestine bacteria may affect the maternal IgG transfer through regulating the breast TLR4 and TLR2 of dams.
登录
查看更多内容
影响因子：
6.2
作者：
Gagnebin, Yoric;Tonoli, David;Rudaz, Serge
通讯作者：
Rudaz, Serge
影响因子：
64.8
作者：
Song, Xinyang;Sun, Ximei;Kasper, Dennis L.
通讯作者：
Kasper, Dennis L.
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
4.4
作者：
Basha S;Surendran N;Pichichero M
通讯作者：
Pichichero M
影响因子：
34.3
作者：
Kotloff, Karen L.;Nasrin, Dilruba;Levine, Myron M.
通讯作者：
Levine, Myron M.