Dynamic evolution of V1R putative pheromone receptors between Mus musculus and Mus spretus.
Dynamic evolution of V1R putative pheromone receptors between Mus musculus and Mus spretus.
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DOI：
10.1186/1471-2164-10-74
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发表时间：
2009-02-09
期刊：
影响因子：
4.4
通讯作者：
Lane RP
中科院分区：
文献类型：
--
作者：
Kurzweil VC;Getman M;NISC Comparative Sequencing Program;Green ED;Lane RP
哺乳动物犁鼻器官（VNO）表达介导信息素反应的两个g蛋白偶联受体基因家族，即V1R和V2R受体基因。在啮齿类动物中，大约有150个V1R基因，包括12个亚家族，在多个染色体位置以基因簇的形式组织。先前，我们发现在小鼠和大鼠谱系的进化分裂期间，这些亚家族中的几个被基因复制、缺失和基因转换广泛调节，这与V1R谱可能参与强化物种形成事件的假设一致。在这里，我们生成了一个包含两个V1R亚家族的大簇的基因组序列，这是一个与小家鼠密切相关的同域物种，并研究了这些基因在两个小鼠谱系中的进化变化。我们根据之前在小鼠和大鼠之间的观察结果，比较了spretus和musus在基因组组织和合成以及V1R基因含量和系统发育方面的差异。与小鼠与大鼠的比较不同，两种小鼠之间的同音性似乎在很大程度上是保守的。局部合成的破坏通常与重复内容的差异有关，尽管这些差异似乎更多地来自删除而不是新的整合。尽管明确的V1R同源性是显而易见的，但我们观察到功能库的动态调节，在spretus中，7个V1Rb基因中的2个和11个V1Ra基因中的1个丢失，两个V1Ra基因在肌肉中成为假基因，另外两个同源对显然受到强适应选择的影响，另一个不同的同源对显然受到基因转换的影响。因此，18个推测的V1Ra/V1Rb基因中的8个（约44%）似乎在这两个物种分化后经历了功能调节。与大鼠-小鼠分裂相比，这两个V1R亚家族的独立扩展明显显示出调节作用，而肌肉和spretus之间的分化更多地是由编码序列中的突变引起的。这些结果支持了功能性V1R谱的适应性变化有助于描述非常密切相关物种的假设。
The mammalian vomeronasal organ (VNO) expresses two G-protein coupled receptor gene families that mediate pheromone responses, the V1R and V2R receptor genes. In rodents, there are ~150 V1R genes comprising 12 subfamilies organized in gene clusters at multiple chromosomal locations. Previously, we showed that several of these subfamilies had been extensively modulated by gene duplications, deletions, and gene conversions around the time of the evolutionary split of the mouse and rat lineages, consistent with the hypothesis that V1R repertoires might be involved in reinforcing speciation events. Here, we generated genome sequence for one large cluster containing two V1R subfamilies in Mus spretus, a closely related and sympatric species to Mus musculus, and investigated evolutionary change in these repertoires along the two mouse lineages. We describe a comparison of spretus and musculus with respect to genome organization and synteny, as well as V1R gene content and phylogeny, with reference to previous observations made between mouse and rat. Unlike the mouse-rat comparisons, synteny seems to be largely conserved between the two mouse species. Disruption of local synteny is generally associated with differences in repeat content, although these differences appear to arise more from deletion than new integrations. Even though unambiguous V1R orthology is evident, we observe dynamic modulation of the functional repertoires, with two of seven V1Rb and one of eleven V1Ra genes lost in spretus, two V1Ra genes becoming pseudogenes in musculus, two additional orthologous pairs apparently subject to strong adaptive selection, and another divergent orthologous pair that apparently was subjected to gene conversion. Therefore, eight of the 18 (~44%) presumptive V1Ra/V1Rb genes in the musculus-spretus ancestor appear to have undergone functional modulation since these two species diverged. As compared to the rat-mouse split, where modulation is evident by independent expansions of these two V1R subfamilies, divergence between musculus and spretus has arisen more by mutations within coding sequences. These results support the hypothesis that adaptive changes in functional V1R repertoires contribute to the delineation of very closely related species.
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DOI：
10.1007/bf01922917
发表时间：
1978-01-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
BONHOMME, F;MARTIN, S;THALER, L
通讯作者：
THALER, L
影响因子：
64.8
作者：
DOBZHANSKY, T;PAVLOVSKY, O
通讯作者：
PAVLOVSKY, O
影响因子：
3.5
作者：
Weiler, E;Apfelbach, R;Farbman, AI
通讯作者：
Farbman, AI
影响因子：
1.7
作者：
Dorries, KM;AdkinsRegan, E;Halpern, BP
通讯作者：
Halpern, BP
影响因子：
64.5
作者：
Herrada, G;Dulac, C
通讯作者：
Dulac, C