Comparative genome analysis of the high pathogenicity Salmonella Typhimurium strain UK-1.
Comparative genome analysis of the high pathogenicity Salmonella Typhimurium strain UK-1.
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DOI：
10.1371/journal.pone.0040645
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Curtiss R 3rd
中科院分区：
文献类型：
--
作者：
Luo Y;Kong Q;Yang J;Mitra A;Golden G;Wanda SY;Roland KL;Jensen RV;Ernst PB;Curtiss R 3rd
肠炎沙门氏菌鼠伤寒血清型是一种革兰氏阴性兼性杆状细菌，可导致沙门氏菌病和食源性疾病，是发达国家和发展中国家最常见的分离出的沙门氏菌血清型之一。几个鼠伤寒沙门氏菌的基因组已经完成，还有更多的基因组测序项目正在进行中。对多个菌株进行比较基因组分析有助于更好地理解鼠伤寒沙门氏菌的进化及其致病机制。鼠伤寒沙门氏菌UK - 1菌株（属于噬菌体1型）经口给予小鼠和鸡时具有高度致病性，并能有效在这些物种的淋巴组织中定殖。这些特性使该菌株成为疫苗研发的良好选择。事实上，UK - 1已被用作许多非重组和重组疫苗菌株的亲本菌株，包括几种家禽用商业疫苗。在这项研究中，我们对UK - 1菌株与其他鼠伤寒沙门氏菌菌株进行了全面的比较基因组分析，并研究了一些基因组差异对表型的影响。全基因组比较凸显了UK - 1菌株与其他鼠伤寒沙门氏菌菌株之间极其密切的关系；然而，也探究了许多UK - 1特有的有趣的遗传和基因组变异。特别是，一个与编码T3SS效应蛋白NleC的基因高度相似的UK - 1特异性基因的缺失，导致其对BALB/c小鼠的口服致病性显著降低。UK - 1中的完整基因组成，特别是那些有助于致病性或有助于确定细菌物种内多样性的元件，为评估重要遗传决定因素的功能特性以及疫苗研发提供了关键信息。
Salmonella enterica serovar Typhimurium, a gram-negative facultative rod-shaped bacterium causing salmonellosis and foodborne disease, is one of the most common isolated Salmonella serovars in both developed and developing nations. Several S. Typhimurium genomes have been completed and many more genome-sequencing projects are underway. Comparative genome analysis of the multiple strains leads to a better understanding of the evolution of S. Typhimurium and its pathogenesis. S. Typhimurium strain UK-1 (belongs to phage type 1) is highly virulent when orally administered to mice and chickens and efficiently colonizes lymphoid tissues of these species. These characteristics make this strain a good choice for use in vaccine development. In fact, UK-1 has been used as the parent strain for a number of nonrecombinant and recombinant vaccine strains, including several commercial vaccines for poultry. In this study, we conducted a thorough comparative genome analysis of the UK-1 strain with other S. Typhimurium strains and examined the phenotypic impact of several genomic differences. Whole genomic comparison highlights an extremely close relationship between the UK-1 strain and other S. Typhimurium strains; however, many interesting genetic and genomic variations specific to UK-1 were explored. In particular, the deletion of a UK-1-specific gene that is highly similar to the gene encoding the T3SS effector protein NleC exhibited a significant decrease in oral virulence in BALB/c mice. The complete genetic complements in UK-1, especially those elements that contribute to virulence or aid in determining the diversity within bacterial species, provide key information in evaluating the functional characterization of important genetic determinants and for development of vaccines.
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