Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.
Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.
复制标题
ATP 激活电流的表型与其神经元中支配牙髓的 P2X1-6 亚基的基因型相关。
DOI：
10.1016/j.bbrc.2015.01.156
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发表时间：
2015
影响因子：
3.1
通讯作者：
Chao
中科院分区：
文献类型：
--
作者：
Yu;X. Tian;Yu;Lin Chen;C. Yi;Zhi;Ying Zhang;Chao
为了探究atp激活电流的表型与痛觉感受器中P2X1-6亚基基因型的关系，我们开发了一种方法，利用逆行荧光示踪法标记大鼠三叉神经节（TG）神经元中支配牙髓的痛觉神经元，在新分离的荧光标记神经元中记录atp激活电流，然后对同一神经元中的P2X1-6亚基进行单细胞免疫组织化学染色。我们发现，将100 μM ATP快速应用于荧光追踪的TG神经元在87%（96/110）的被测细胞中产生强大的内向电流。细胞直径为16 ~ 56 μm。记录三种atp激活电流（F、I、S），且电流上升次数明显（r10 ~ 90, P < 0.05）。细胞直径与R10-90值呈正相关（P < 0.05）， R10-90值随细胞直径的增加而增加。F型电流下对ATP有反应的细胞P2X3和P2X5主要呈阳性，P2X2呈阴性；I型电流对ATP有反应的细胞P2X1-3和P2X5呈阳性，P2X4呈阴性；S型电流对ATP有反应的细胞P2X1-5呈阳性，P2X6呈阴性。本研究结果表明，除了P2X3亚基外，P2X5亚基也参与了小尺寸伤害性神经元中F型atp激活电流的产生。除了含有P2X2/3亚基的通道外，天然中等伤害性神经元中还存在更复杂的P2X1-5亚基的非特征组合，表现为I和S型atp激活电流。此外，在支配牙髓的大鼠TG神经元中，P2X6亚基并不是参与伤害性信号的主要亚基。
To explore the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in nociceptors, we developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion (TG) neurons using a retrograde fluorescence-tracing method, to record ATP-activated current in freshly isolated fluorescence-labeled neurons, and then to conduct single cell immunohistochemical staining for P2X1-6 subunits in the same neuron. We found that fast application of 100 μM ATP to fluorescence-traced TG neurons produced robust inward current in 87% (96/110) of cells tested. The diameter of cells varied from 16 to 56 μm. Three types of ATP-activated current (F, I and S) were recorded with distinct rise times of the current (R10–90, P < 0.05). There was a positive correlation between the cell diameter and the value of R10–90(P < 0.05): the value of R10-90increased with increases in the cell diameter. Cells responsive to ATP with the type F current mainly showed positive staining for P2X3 and P2X5, but negative staining for P2X2; cells responsive to ATP with the type I current showed positive staining for P2X1-3 and P2X5, but negative staining for P2X4; and cells responsive to ATP with the type S current showed positive staining for P2X1-5, but negative staining for P2X6. The present findings suggest that in addition to P2X3 subunits, P2X5 subunits are also involved in the generation of the F type of ATP-activated current in small-sized nociceptive neurons. In addition to the P2X2/3 subunit-containing channels, more complex uncharacterized combinations of P2X1-5 subunits exist in native medium-sized nociceptive neurons exhibiting the I and S types of ATP-activated current. In addition, the P2X6 subunit is not a main subunit involved in the nociceptive signal in rat TG neurons innervating tooth-pulp.
P2X1 和 P2X5 受体亚基的共表达揭示了一种新型 ATP 门控离子通道。
影响因子：
3.6
作者：
Torres,GE;Haines,WR;Egan,TM;Voigt,MM
通讯作者：
Voigt,MM
影响因子：
3.5
作者：
Kaczmarek-Hajek, Karina;Loerinczi, Eva;Hausmann, Ralf;Nicke, Annette
通讯作者：
Nicke, Annette