Improvements of Glucose and Lipid Metabolism After Jejuno-ileal Circuit Procedure in a Non-obese Diabetic Rat Model
Improvements of Glucose and Lipid Metabolism After Jejuno-ileal Circuit Procedure in a Non-obese Diabetic Rat Model
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非肥胖糖尿病大鼠模型空肠回肠回路手术后葡萄糖和脂质代谢的改善
DOI：
10.1007/s11695-015-1997-8
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发表时间：
2016-08
期刊：
影响因子：
2.9
通讯作者：
Hu Sanyuan
中科院分区：
文献类型：
--
作者：
Wang Yanmin;Zhang Xiang;Zhong Mingwei;Liu Teng;Zhang Guangyong;Liu Shaozhuang;Guo Wei;Wei Meng;He Qingsi;Sun Dong;Hu Sanyuan
背景在最近的一项研究中，我们发现空回肠循环（JIC）手术可以有效地改善葡萄糖稳态，但其内在机制需要进一步研究。此外，JIC在脂质代谢中的作用也是未知的。鉴于肥胖会增加胰岛素敏感性，我们假设JIC手术改善了脂肪代谢，从而进一步有助于糖尿病缓解。本研究的目的是探讨JIC手术对非肥胖糖尿病大鼠模型脂代谢和糖稳态的影响。方法14只高脂饮食和低剂量链脲佐菌素诱导的糖尿病大鼠随机分为JIC组和假JIC组。测量体重、摄食量、葡萄糖耐量、胰岛素抵抗、血脂参数、胰高血糖素样肽1（GLP-1）和脂肪源性激素。术后12周，用Western blot法检测肝脂肪酸合成酶（FAS）和乙酰辅酶A羧化酶（ACC）的表达。苏木精-伊红染色和油红O染色检测肝脏脂质含量。肠内分泌细胞在回肠远端进行了检查，通过免疫组化staining.ResultsRelative的假手术组，JIC大鼠表现出显着改善葡萄糖耐量，胰岛素抵抗，血脂异常，体重减轻，GLP-1和脂联素增加，瘦素减少。JIC还降低了FAS和ACC在肝脏中的表达，表现出改善肝脏脂肪含量，并提高GLP-1和嗜铬粒蛋白A在远端gust.ConclusionsJIC水平减轻高血糖大鼠的脂肪代谢紊乱，这可能有助于改善胰岛素敏感性和血糖控制。
BackgroundIn a recent study, we showed a jejuno-ileal circuit (JIC) procedure that effectively improved glucose homeostasis, but the intrinsic mechanism requires further studies. Furthermore, the role of JIC in lipid metabolism is also unknown. Given that adiposity aggravates insulin sensitivity, we hypothesize that the JIC procedure improves fat metabolism and thus further contributes to diabetic remission. The aim of this study was to investigate the effects of JIC surgery on lipid metabolism and glucose homeostasis in a non-obese diabetic rat model.MethodsFourteen high-fat diet and low-dose streptozotocin-induced diabetic rats were randomly divided into JIC and sham-JIC groups. Body weight, food intake, glucose tolerance, insulin resistance, serum lipid parameters, glucagon-like peptide 1 (GLP-1), and adipose-derived hormones were measured. At 12 weeks postoperatively, the expressions of hepatic fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) were measured by Western blot. The lipid content of liver was assessed by hematoxylin-eosin staining and Oil Red O staining. The enteroendocrine cells in the distal ileum were examined by immunohistochemical staining.ResultsRelative to the sham group, the JIC rats exhibited significant improvements in glucose tolerance, insulin resistance, and dyslipidemia without weight loss, showing increased GLP-1 and adiponectin and decreased leptin. JIC also reduced the expression of FAS and ACC in the liver, exhibited improved hepatic fat content, and raised the levels of GLP-1 and chromogranin A in the distal gut.ConclusionsJIC alleviated lipometabolic disorders in hyperglycemic rats, which may contribute to the amelioration of insulin sensitivity and glycemic control.
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影响因子：
9
作者：
Jimenez, Amanda;Casamitjana, Roser;Vidal, Josep
通讯作者：
Vidal, Josep
DOI：
10.1038/oby.2009.102
发表时间：
2009-09
期刊：
Obesity (Silver Spring, Md.)
影响因子：
--
作者：
Patti ME;Houten SM;Bianco AC;Bernier R;Larsen PR;Holst JJ;Badman MK;Maratos-Flier E;Mun EC;Pihlajamaki J;Auwerx J;Goldfine AB
通讯作者：
Goldfine AB
roux-en-y胃旁路对具有正常葡萄糖耐受性和2型糖尿病肥胖受试者的肥胖受试者的禁食和餐后炎症参数的影响。
影响因子：
4.8
作者：
Lindegaard KK;Jorgensen NB;Just R;Heegaard PM;Madsbad S
通讯作者：
Madsbad S
DOI：
10.1210/jcem.87.7.8638
发表时间：
2002-07
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Anneli Seppälä‐Lindroos;S. Vehkavaara;A. Häkkinen;Takashi Goto;J. Westerbacka;Anssi Sovijärvi;Juha Halav
通讯作者：
Anneli Seppälä‐Lindroos;S. Vehkavaara;A. Häkkinen;Takashi Goto;J. Westerbacka;Anssi Sovijärvi;Juha Halav
影响因子：
168.9
作者：
Weiss, R;Dufour, S;Caprio, S
通讯作者：
Caprio, S