Enhanced cytotoxicity for colon 26 cells using doxorubicin-loaded sorbitan monooleate (Span 80) vesicles.
Enhanced cytotoxicity for colon 26 cells using doxorubicin-loaded sorbitan monooleate (Span 80) vesicles.
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DOI：
10.7150/ijbs.5453
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发表时间：
2013
影响因子：
9.2
通讯作者：
Umakoshi H
中科院分区：
文献类型：
--
作者：
Hayashi K;Tatsui T;Shimanouchi T;Umakoshi H
Span 80（脱水山梨糖醇单油酸酯）囊泡的行为不同于传统的磷脂囊泡（脂质体），因为前者有一个更多的流体界面。在将盐酸阿霉素（DOX）包封到Span 80囊泡中（装载效率：63%）之后，将装载有DOX的Span 80囊泡（DV）加入到Colon 26细胞中。流式细胞术分析和激光共聚焦显微镜观察表明，DVs可直接将DOX导入结肠26细胞的胞浆。DV表现出与载阿霉素脂质体（DL）不同的递送方式。认为DV和DL之间递送方式的差异导致细胞毒性（IC 50）的差异;即DV和DL的IC 50值分别为5和> 30 μM。本文所获得的结果将提供基本的发现，这可以有助于改进传统的脂质体为基础的载体的配方和其细胞毒性。
Span 80 (sorbitan monooleate) vesicles behaved differently from conventional phospholipid vesicles (liposomes) because the former had a more fluid interface. After doxorubicin hydrochloride (DOX) was encapsulated into the Span 80 vesicle (loading efficiency: 63 %), DOX-loaded Span 80 vesicles (DVs) were thereafter added to Colon 26 cells. It was suggested, from the flow cytometric analysis and confocal laser microscopic observation, that DVs directly deliver DOX into the cytoplasm of Colon 26 cells. DVs showed the different delivery manner from the DOX-loaded liposomes (DLs). It is considered that the difference of delivery manner between DVs and DLs resulted in the difference of cytotoxicity (IC50); i.e. IC50 values for DVs and DLs were 5 and > 30 μM, respectively. The results obtained herein would give the fundamental findings which can contribute to the improvement of formulation of conventional liposome-based carrier and its cytotoxicity.
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影响因子：
17.1
作者：
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通讯作者：
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影响因子：
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