An evolutionary approach uncovers a diverse response of tRNA 2-thiolation to elevated temperatures in yeast.
An evolutionary approach uncovers a diverse response of tRNA 2-thiolation to elevated temperatures in yeast.
复制标题
DOI：
10.1261/rna.048199.114
复制
发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Leidel SA
中科院分区：
文献类型：
--
作者：
Alings F;Sarin LP;Fufezan C;Drexler HC;Leidel SA
转运RNA（tRNA）分子的化学修饰在进化上是保守的，对翻译和tRNA结构至关重要。很少有人知道这些核苷修饰如何响应生理应激。使用质谱和互补的方法，我们定义了tRNA的修饰水平在6种酵母菌的温度升高。我们发现，在常用的酿酒酵母实验室菌株S288 C和W303以及贝酵母中，在超过30°C的温度下，34位尿苷（s2 U34）的2-巯基化受损。应力缓解后，巯基化水平恢复，我们没有发现修饰的tRNA或s2 U34核苷被主动去除的证据。我们的研究结果表明，2-巯基化的损失后积累的新合成的tRNA，缺乏s2 U34修饰，由于温度敏感性的URM 1途径在S。酿酒酵母和酿酒酵母。bayanus此外，我们对选定酵母物种中tRNA修饰模式的分析揭示了两种替代表型。大多数菌株适度增加其tRNA修饰水平，以响应热，可能构成了对高温的共同适应。然而，核苷修饰的总体减少仅在S288 C中观察到。这一令人惊讶的发现强调了利用进化生物学力量进行研究的重要性，并强调了未来在其他模式生物中对tRNA修饰进行系统研究的必要性。
Chemical modifications of transfer RNA (tRNA) molecules are evolutionarily well conserved and critical for translation and tRNA structure. Little is known how these nucleoside modifications respond to physiological stress. Using mass spectrometry and complementary methods, we defined tRNA modification levels in six yeast species in response to elevated temperatures. We show that 2-thiolation of uridine at position 34 (s2U34) is impaired at temperatures exceeding 30°C in the commonly used Saccharomyces cerevisiae laboratory strains S288C and W303, and in Saccharomyces bayanus. Upon stress relief, thiolation levels recover and we find no evidence that modified tRNA or s2U34 nucleosides are actively removed. Our results suggest that loss of 2-thiolation follows accumulation of newly synthesized tRNA that lack s2U34 modification due to temperature sensitivity of the URM1 pathway in S. cerevisiae and S. bayanus. Furthermore, our analysis of the tRNA modification pattern in selected yeast species revealed two alternative phenotypes. Most strains moderately increase their tRNA modification levels in response to heat, possibly constituting a common adaptation to high temperatures. However, an overall reduction of nucleoside modifications was observed exclusively in S288C. This surprising finding emphasizes the importance of studies that utilize the power of evolutionary biology, and highlights the need for future systematic studies on tRNA modifications in additional model organisms.
登录
查看更多内容
影响因子：
11.4
作者：
EMILSSON, V;KURLAND, CG
通讯作者：
KURLAND, CG
影响因子：
4.5
作者：
Grosjean H;Breton M;Sirand-Pugnet P;Tardy F;Thiaucourt F;Citti C;Barré A;Yoshizawa S;Fourmy D;de Crécy-Lagard V;Blanchard A
通讯作者：
Blanchard A
影响因子：
16
作者：
Begley, Ulrike;Dyavaiah, Madhu;Begley, Thomas J.
通讯作者：
Begley, Thomas J.
影响因子：
3.3
作者：
Iwasaki O;Tanaka A;Tanizawa H;Grewal SI;Noma K
通讯作者：
Noma K
影响因子：
16.6
作者：
通讯作者：
--