Acoustic Detection of Retained Perfluoropropane Droplets Within the Developing Myocardial Infarct Zone.
Acoustic Detection of Retained Perfluoropropane Droplets Within the Developing Myocardial Infarct Zone.
复制标题
DOI：
10.1016/j.ultrasmedbio.2022.07.005
复制
发表时间：
2022-11
影响因子：
2.9
通讯作者：
Porter, Thomas
中科院分区：
文献类型：
--
作者：
Zeng, Ping;Chen, Cheng;Lof, John;Stolze, Elizabeth;LI, Shouqiang;Chen, Xucai;Pacella, John;Villanueva, Flordeliza S.;Matsunaga, Terry;Everbach, E. Carr;Fei, Hongwen;Xie, Feng;Porter, Thomas
关键词：
全氟丙烷液滴（PD）穿过内皮屏障，可被声学激活，用于静脉注射（IVI）后的选择性心肌血管外增强。我们的目的是确定如何最佳激活血管外PD，以经胸超声增强描绘正在形成的瘢痕区（DSZ）。超快帧率显微镜（UFRM）进行，以确定脉冲序列的影响，从PD气泡形成的阈值。随后在不同流速下进行体外研究，以使用多脉冲基波非线性（FNLI）或单脉冲谐波成像（SPHI）确定PD输注的声激活和惯性空化（IC）阈值。在9只大鼠和10只猪的左前降支长时间缺血后给予PD IVI，以检测和量化DSZ内的PD动力学。一个多脉冲序列有一个较低的MI阈值的声学激活UFRM。对于两个脉冲序列，在低于IC阈值的MI> 0.4时观察到声激活。在大鼠中，共聚焦显微镜和连续声激活成像检测到较高的液滴存在（相对于远程区域）内的DSZ在3分钟后IVI。IVI后此时，猪的经胸高MI脉冲和FNLI导致DSZ内的选择性对比度增强。
Perfluoropropane droplets (PD) cross endothelial barriers and can be acoustically activated for selective myocardial extravascular enhancement following intravenous injection (IVI). Our objective was to determine how to optimally activate extravascular PD for transthoracic ultrasound-enhanced delineation of a developing scar zone (DSZ). Ultrafast frame rate microscopy (UFRM) was conducted to determine the effect of pulse sequence on the threshold of bubble formation from PDs. In vitro studies were subsequently performed at different flow rates to determine acoustic activation and inertial cavitation (IC) thresholds for a PD infusion using multipulse fundamental non-linear (FNLI) or single pulse harmonic imaging (SPHI). IVI of PDs were given in nine rats and 10 pigs following prolonged left anterior descending ischemia to detect and quantify PD kinetics within the DSZ. A multipulse sequence had a lower MI threshold for acoustic activation by UFRM. Acoustic activation was observed at an MI of > 0.4 below the IC threshold for both pulse sequences. In rats, confocal microscopy and serial acoustic activation imaging detected higher droplet presence (relative to remote regions) within the DSZ at 3 minutes post IVI. Transthoracic high MI impulses with FNLI in pigs at this time post IVI resulted in selective contrast enhancement within the DSZ.
登录
查看更多内容
DOI：
10.1073/pnas.1606915113
发表时间：
2016-09-06
影响因子：
11.1
作者：
Helfield, Brandon;Chen, Xucai;Villanueva, Flordeliza S.
通讯作者：
Villanueva, Flordeliza S.
影响因子：
12.4
作者：
Matsunaga TO;Sheeran PS;Luois S;Streeter JE;Mullin LB;Banerjee B;Dayton PA
通讯作者：
Dayton PA
影响因子：
1.6
作者：
Chen, Xucai;Wang, Jianjun;Villanueva, Flordeliza S.
通讯作者：
Villanueva, Flordeliza S.
影响因子：
20.1
作者：
Jones, Steven P.;Tang, Xian-Liang;Bolli, Roberto
通讯作者：
Bolli, Roberto
影响因子：
2.9
作者：
Burgess MT;Porter TM
通讯作者：
Porter TM