Collaborative Ocular Oncology Group report number 1: prospective validation of a multi-gene prognostic assay in uveal melanoma.
Collaborative Ocular Oncology Group report number 1: prospective validation of a multi-gene prognostic assay in uveal melanoma.
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DOI：
10.1016/j.ophtha.2012.02.017
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发表时间：
2012-08
期刊：
影响因子：
13.7
通讯作者：
Harbour JW
中科院分区：
文献类型：
--
作者：
Onken MD;Worley LA;Char DH;Augsburger JJ;Correa ZM;Nudleman E;Aaberg TM Jr;Altaweel MM;Bardenstein DS;Finger PT;Gallie BL;Harocopos GJ;Hovland PG;McGowan HD;Milman T;Mruthyunjaya P;Simpson ER;Smith ME;Wilson DJ;Wirostko WJ;Harbour JW
这项研究评估了15个基因表达谱（GEP）测定的预后表现，将原发性葡萄膜后黑色素瘤划分为预后亚组：1级（低转移风险）和2级（高转移风险）。前瞻性、多中心研究。来自12个独立中心的459例葡萄膜后黑色素瘤患者入组。GEP将肿瘤分为1级和2级。用单核苷酸多态性测定法分析了前260个样本的3号染色体状态。进行净重分类改进分析，比较GEP与第7版临床肿瘤-淋巴结-转移（TNM）分类和3号染色体状态的预后准确性。对患者的原发肿瘤进行治疗，并监测转移情况。GEP试验成功分类446/459例（97.2%）。GEP为1级276例（61.9%），2级170例（38.1%）。中位随访时间为17.4个月（平均18.0个月）。1级3例（1.1%）、2级44例（25.9%）存在转移（log rank检验，P<10−14）。虽然GEP 2级与3号单体存在相关性（Fisher精确检验，P<0.0001），但54/260例（20.8%）肿瘤的GEP与3号染色体状态不一致，其中GEP表现出更高的预后准确性（log rank检验，P=0.0001）。通过多变量Cox模型，与其他预后因素相比，GEP分级与转移的独立相关性更强（P<0.0001）。3号染色体状态不提供与GEP无关的其他预后信息（P=0.2）。在三年的随访中，GEP比TNM分类的净重分类改善为0.43 (P=0.001)，比3号染色体状态的净重分类改善为0.38 （P=0.004）。GEP试验具有很高的技术成功率，是所有分析因素中最准确的预后标志物。与临床TNM分类和3号染色体状态相比，GEP在预后准确性方面提供了非常显著的改善。3号染色体状态不能提供与GEP无关的预后信息。
This study evaluates the prognostic performance of a 15 gene expression profiling (GEP) assay that assigns primary posterior uveal melanomas to prognostic subgroups: class 1 (low metastatic risk) and class 2 (high metastatic risk). Prospective, multicenter study. 459 patients with posterior uveal melanoma were enrolled from 12 independent centers. Tumors were classified by GEP as class 1 or class 2. The first 260 samples were also analyzed for chromosome 3 status using a single nucleotide polymorphism assay. Net reclassification improvement analysis was performed to compare the prognostic accuracy of GEP to the 7th edition clinical Tumor-Node-Metastasis (TNM) classification and to chromosome 3 status. Patients were managed for their primary tumor and monitored for metastasis. The GEP assay successfully classified 446/459 (97.2%) cases. The GEP was class 1 in 276 cases (61.9%) and class 2 in 170 cases (38.1%). Median follow-up was 17.4 months (mean, 18.0 months). Metastasis was detected in 3 (1.1%) class 1 cases and 44 (25.9%) class 2 cases (log rank test, P<10−14). Although there was an association between GEP class 2 and monosomy 3 (Fisher exact test, P<0.0001), 54/260 (20.8%) tumors were discordant for GEP and chromosome 3 status, among which GEP demonstrated superior prognostic accuracy (log rank test, P=0.0001). Using multivariate Cox modeling, GEP class had a stronger independent association with metastasis than any other prognostic factor (P<0.0001). Chromosome 3 status did not contribute additional prognostic information that was independent of GEP (P=0.2). At three years follow-up, the net reclassification improvement of GEP over TNM classification was 0.43 (P=0.001) and 0.38 (P=0.004) over chromosome 3 status. The GEP assay had a high technical success rate and was the most accurate prognostic marker among all of the factors analyzed. GEP provided a highly significant improvement in prognostic accuracy over clinical TNM classification and chromosome 3 status. Chromosome 3 status did not provide prognostic information that was independent of GEP.
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影响因子：
3.7
作者：
SISLEY, K;RENNIE, IG;REES, RC
通讯作者：
REES, RC
影响因子：
13.7
作者：
Young, Tara A.;Rao, Nagesh P.;Straatsma, Bradley R.
通讯作者：
Straatsma, Bradley R.
影响因子：
2
作者：
Pencina, Michael J.;D'Agostino, Ralph B., Sr.;Steyerberg, Ewout W.
通讯作者：
Steyerberg, Ewout W.
DOI：
10.1126/science.1194472
发表时间：
2010-12-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harbour JW;Onken MD;Roberson ED;Duan S;Cao L;Worley LA;Council ML;Matatall KA;Helms C;Bowcock AM
通讯作者：
Bowcock AM
影响因子：
11.2
作者：
Onken, MD;Worley, LA;Harbour, JW
通讯作者：
Harbour, JW