The relationship between cyclooxygenase-2 expression and characteristics of malignant transformation in human colorectal adenomas
The relationship between cyclooxygenase-2 expression and characteristics of malignant transformation in human colorectal adenomas
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人结直肠腺瘤中环氧合酶2表达与恶变特征的关系
DOI：
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
E. Kay
中科院分区：
文献类型：
--
作者：
K. Sheehan;F. O'Connell;A. O'Grady;R. Conroy;M. Leader;M. Byrne;F. Murray;E. Kay
背景和目的 环氧合酶 2 (COX-2) 是阿司匹林和其他非甾体抗炎药的靶标，与结直肠癌的发病机制有关。本研究的目的是评估恶变前结直肠息肉中 COX-2 的程度，并评估 COX-2 与这些病变中不典型增生水平之间的关系。方法通过内镜或手术切除123例患者的完整息肉切除标本。福尔马林固定和石蜡包埋后，对息肉进行组织学评估，以确定不典型增生的大小、类型和等级。使用单克隆 COX-2 抗体，通过亲和素-生物素免疫组织化学技术测量 COX-2 表达的程度。 COX-2表达的程度根据上皮COX-2表达百分比进行分级。结果息肉的组织学类型为：增生性10个，管状腺瘤35个，管状绒毛状腺瘤61个，绒毛状腺瘤17个。 20 例显示轻度不典型增生，65 例显示中度不典型增生，28 例显示局灶性或重度不典型增生（其中 8 例伴有局灶性浸润）。息肉的平均大小为1.7厘米。 9 例增生性息肉为 COX-2 阴性，1 例为 COX-2 阳性。 COX-2 表达在较大的息肉和具有较高绒毛成分的息肉中更为广泛。随着不典型增生严重程度的增加，COX-2蛋白的范围显着增加。在息肉内，上皮内 COX-2 表达出现局灶性相应增加，显示出更高级别的不典型增生。结论 COX-2的表达与结直肠腺瘤性息肉的大小、不典型增生的类型和分级直接相关。这表明COX-2在结直肠癌中的作用可能处于腺瘤到癌序列的早期阶段，并支持抑制COX-2可能有助于对该疾病进行化学预防的建议。
Background and aims Cyclooxygenase 2 (COX-2) is a target of aspirin and other non-steroidal anti-inflammatory drugs and is implicated in the pathogenesis of colorectal cancer. The objective of this study was to evaluate the extent of COX-2 in pre-malignant colorectal polyps and to assess the relationship between COX-2 and the level of dysplasia in these lesions. Methods Whole polypectomy specimens were retrieved from 123 patients by endoscopic or surgical resection. Following formalin fixation and paraffin embedding, the polyps were evaluated histologically for size, type and grade of dysplasia. The extent of COX-2 expression was measured by the avidin–biotin immunohistochemical technique using a monoclonal COX-2 antibody. The extent of COX-2 expression was graded according to percentage epithelial COX-2 expression. Results The polyps were of the following histological types: 10 hyperplastic, 35 tubular adenomas, 61 tubulovillous adenomas and 17 villous adenomas. Twenty showed mild dysplasia, 65 moderate dysplasia, and 28 focal or severe dysplasia (including eight with focal invasion). The average polyp size was 1.7 cm. Nine hyperplastic polyps were COX-2-negative and one was COX-2-positive. COX-2 expression was more extensive in larger polyps and in polyps with a higher villous component. There was a significant increase in the extent of COX-2 protein with increasing severity of dysplasia. Within a polyp, there was a focal corresponding increase in COX-2 expression within epithelium showing a higher grade of dysplasia. Conclusions COX-2 expression is related directly to colorectal adenomatous polyp size, type and grade of dysplasia. This suggests that the role of COX-2 in colorectal cancer may be at an early stage in the adenoma-to-carcinoma sequence and supports the suggestion that inhibition of COX-2 may be useful chemoprevention for this disease.
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影响因子：
11.2
作者：
Vincent W. Yang;J. M. Shields;Stanley R. Hamilton;Ernst W. Spannhake;Walter C. Hubbard;L. Hylind;C. Robinson;F. Giardiello
通讯作者：
Vincent W. Yang;J. M. Shields;Stanley R. Hamilton;Ernst W. Spannhake;Walter C. Hubbard;L. Hylind;C. Robinson;F. Giardiello
影响因子：
29.4
作者：
Williams, CS;Luongo, C;DuBois, RN
通讯作者：
DuBois, RN
选择性环氧合酶 2 抑制剂可抑制 H-ras 转化的大鼠肠上皮细胞的生长。
影响因子：
29.4
作者：
Sheng,GG;Shao,J;Sheng,H;Hooton,EB;Isakson,PC;Morrow,JD;CoffeyJr,RJ;DuBois,RN;Beauchamp,RD
通讯作者：
Beauchamp,RD
影响因子：
11.2
作者：
W. Karnes;R. Shattuck-Brandt;L. Burgart;R. Dubois;D. Tester;J. Cunningham;C. Y. Kim;S. McDonnell-S.-McDonne
通讯作者：
W. Karnes;R. Shattuck-Brandt;L. Burgart;R. Dubois;D. Tester;J. Cunningham;C. Y. Kim;S. McDonnell-S.-McDonne
影响因子：
11.2
作者：
H. Sano;Y. Kawahito;R. Wilder;A. Hashiramoto;S. Mukai;K. Asai;S. Kimura;H. Kato;M. Kondo;T. Hla
通讯作者：
H. Sano;Y. Kawahito;R. Wilder;A. Hashiramoto;S. Mukai;K. Asai;S. Kimura;H. Kato;M. Kondo;T. Hla