Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow.
Convenient structural analysis of glycosphingolipids using MALDI-QIT-TOF mass spectrometry with increased laser power and cooling gas flow.
复制标题
使用 MALDI-QIT-TOF 质谱法并增加激光功率和冷却气流，可方便地对鞘糖脂进行结构分析。
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
A. Suzuki
中科院分区：
文献类型：
--
作者：
Yusuke Suzuki;Minoru Suzuki;Emi Ito;Naoko Goto;K. Miseki;J. Iida;Yuzo Yamazaki;Masaki Yamada;A. Suzuki
采用基质辅助激光解吸/电离四极离子阱飞行时间质谱法（MALDI-QIT-TOF MS）对中性鞘糖脂进行了结构表征。采用MALDI-QIT-TOF质谱仪，在激光功率增大和冷却气体流量增大的条件下，对鞘糖脂的锂加合物离子进行了分析。在较强的条件下，碎片离子的相对强度增加，所得光谱显示鞘脂中存在碎片化的低聚糖离子。因此，很容易得到鞘糖脂的低聚糖序列。为了获得更详细的结构信息，分别对乳糖神经酰胺和神经酰胺离子进行MS/MS （MS2）和MS/MS/MS （MS3）分析。所得数据足以确定每种鞘糖脂的低聚糖和神经酰胺部分的结构。强条件下MS2和MS3对Forssman糖脂的裂解模式与标准条件下MS3和MS4的裂解模式相当。因此，增加激光功率和冷却气体流量的MALDI-QIT-TOF质谱是一种简便的糖鞘脂分析方法。
Matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) was applied to the structural characterization of neutral glycosphingolipids. Lithium adduct ions of glycosphingolipids were analyzed using MALDI-QIT-TOF MS under strong conditions of increased laser power and cooling gas flow. The relative intensities of fragment ions were increased under the strong conditions, and the resulting spectra revealed the presence of oligosaccharide ions fragmented from the glycosphingolipids. Consequently, the oligosaccharide sequences of the glycosphingolipids were readily obtained. To obtain more detailed structural information, MS/MS (MS2) and MS/MS/MS (MS3) analyses were performed with selection of the lactosylceramide and ceramide ions, respectively. The resulting data were sufficient to determine the structures of both the oligosaccharide and the ceramide moiety of each glycosphingolipid. The fragmentation patterns of MS2 and MS3 for Forssman glycolipid under the strong conditions were comparable to those of MS3 and MS4 obtained under standard conditions, respectively. Thus, MALDI-QIT-TOF MS with increased laser power and cooling gas flow is a convenient method for glycosphingolipid analysis.
影响因子：
2.9
作者：
DOMON, B;COSTELLO, CE
通讯作者：
COSTELLO, CE
DOI：
10.1002/(sici)1097-0231(20000415)14:7
发表时间：
2000-01-01
影响因子：
2
作者：
Levery, SB;Toledo, MS;Takahashi, HK
通讯作者：
Takahashi, HK