A novel malaria vaccine candidate antigen expressed in Tetrahymena thermophila.
A novel malaria vaccine candidate antigen expressed in Tetrahymena thermophila.
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DOI：
10.1371/journal.pone.0087198
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cavanagh DR
中科院分区：
文献类型：
--
作者：
Cowan GJ;Bockau U;Eleni-Muus J;Aldag I;Samuel K;Creasey AM;Hartmann MW;Cavanagh DR
有效疟疾疫苗的开发受到生产正确折叠的疟原虫蛋白用作疫苗组分的问题的阻碍。我们已经研究了一种新的纤毛虫表达系统，嗜热四膜虫，作为恶性疟原虫疫苗抗原平台的使用。在嗜热四膜虫中表达由裂殖子表面蛋白-1（MSP-1）的N-末端和C-末端组成的合成疫苗抗原。将重组抗原分泌到培养基中，并通过单克隆抗体（mAb）亲和层析纯化。该疫苗在MF 1小鼠中具有免疫原性，引起针对N-和C-末端组分的高抗体滴度。免疫动物的血清与来自三种抗原性不同菌株的恶性疟原虫寄生虫通过免疫荧光测定强烈反应，证实产生的抗体能够识别其天然形式的寄生虫抗原。用源自所有三种MSP-1 Block 2血清型的肽文库进行的血清反应性的表位作图证实，疫苗的MSP-1 Block 2杂合组分有效地靶向MSP-1的该多态性区域的所有三种血清型。本研究成功地证明了嗜热四膜虫作为重组蛋白表达平台用于生产疟疾疫苗抗原的用途。
Development of effective malaria vaccines is hampered by the problem of producing correctly folded Plasmodium proteins for use as vaccine components. We have investigated the use of a novel ciliate expression system, Tetrahymena thermophila, as a P. falciparum vaccine antigen platform. A synthetic vaccine antigen composed of N-terminal and C-terminal regions of merozoite surface protein-1 (MSP-1) was expressed in Tetrahymena thermophila. The recombinant antigen was secreted into the culture medium and purified by monoclonal antibody (mAb) affinity chromatography. The vaccine was immunogenic in MF1 mice, eliciting high antibody titers against both N- and C-terminal components. Sera from immunized animals reacted strongly with P. falciparum parasites from three antigenically different strains by immunofluorescence assays, confirming that the antibodies produced are able to recognize parasite antigens in their native form. Epitope mapping of serum reactivity with a peptide library derived from all three MSP-1 Block 2 serotypes confirmed that the MSP-1 Block 2 hybrid component of the vaccine had effectively targeted all three serotypes of this polymorphic region of MSP-1. This study has successfully demonstrated the use of Tetrahymena thermophila as a recombinant protein expression platform for the production of malaria vaccine antigens.
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影响因子：
1.5
作者：
Adda, Christopher G.;Murphy, Vince J.;Sunde, Margaret;Waddington, Lynne J.;Schloegel, Jesse;Talbo, Gert H.;Vingas, Kleo;Kienzle, Vivian;Masciantonio, Rosella;Howlett, Geoffrey J.;Hodder, Anthony N.;Foley, Michael;Anders, Robin F.
通讯作者：
Anders, Robin F.
影响因子：
15.9
作者：
Crompton, Peter D.;Pierce, Susan K.;Miller, Louis H.
通讯作者：
Miller, Louis H.
影响因子：
6.4
作者：
Egan, AF;Morris, J;Riley, EM
通讯作者：
Riley, EM
影响因子：
2.2
作者：
Egan, AF;Burghaus, P;Riley, EM
通讯作者：
Riley, EM
DOI：
10.1084/jem.172.1.379
发表时间：
1990-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Blackman MJ;Heidrich HG;Donachie S;McBride JS;Holder AA
通讯作者：
Holder AA