Distribution of an ankyrin-repeat protein on the endoplasmic reticulum in Arabidopsis.
Distribution of an ankyrin-repeat protein on the endoplasmic reticulum in Arabidopsis.
复制标题
拟南芥内质网上锚蛋白重复蛋白的分布。
DOI：
10.1111/j.1744-7909.2008.00791.x
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发表时间：
2009-02
影响因子：
11.4
通讯作者：
中科院分区：
文献类型：
--
作者：
植物细胞中存在着大量的锚蛋白重复蛋白。然而，这些蛋白质的分布和功能大多不清楚。通过逆转录-聚合酶链式反应，从拟南芥中克隆了一个编码Anyrin类蛋白的基因，命名为AtANK1(GenBank登录号：NM_120340)。亲和纯化6-His标记的AtAnk1-N融合蛋白，获得其兔多克隆抗体。纯化的抗AtAnk1-N多克隆抗体免疫印迹显示，AtANK1蛋白的相对分子质量约为76 kDa。用纯化的抗AtAnk1-N多克隆抗体进行免疫荧光标记和免疫金标记，结合共聚焦和透射电子显微镜观察，发现AtANK1分布在拟南芥细胞的内质网膜上。根据这些结果，我们推测AtANK1可能参与了内质网相关蛋白在植物细胞中的定位和分类。
There are many ankyrin-repeat proteins in plant cells. However, the distribution and function of these proteins are mostly unclear. By reverse transcription-polymerase chain reaction, a gene encoding an ankyrin-like protein was cloned from Arabidopsis and named AtANK1 (GenBank accession no. NM_120340). The 6-His-tagged AtAnk1-N fusion protein was affinity-purified and its rabbit polyclonal antibody was obtained. Immuno-blotting with the purified anti-AtAnk1-N polyclonal antibody revealed that the relative molecular weight of the AtANK1 protein was about 76 kDa. By immunofluorescence labeling and immuno-gold labeling with the purified anti-AtAnk1-N polyclonal antibody, coupled with confocal and transmission electron microscopy observation, AtANK1 was found to be distributed on the membrane of the endoplasmic reticulum in Arabidopsis cells. Based on these results, we suggested that AtANK1 might be involved in endoplasmic reticulum-related protein localization and sorting in plant cells.
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影响因子：
4.8
作者：
Kuhlmann, M;Horvay, K;Dröge-Laser, W
通讯作者：
Dröge-Laser, W
影响因子：
7.4
作者：
H. Kuriyama;H. Takano;L. Suzuki;H. Uchida;S. Kawano;H. Kuroiwa;T. Kuroiwa
通讯作者：
H. Kuriyama;H. Takano;L. Suzuki;H. Uchida;S. Kawano;H. Kuroiwa;T. Kuroiwa
影响因子：
4.3
作者：
K. Bosch;E. Newcomb
通讯作者：
K. Bosch;E. Newcomb
DOI：
10.1105/tpc.4.12.1575
发表时间：
1992-12
期刊：
The Plant cell
影响因子：
--
作者：
Hong Zhang;D. C. Scheirer;William H. Fowle;Howard M. Goodman
通讯作者：
Hong Zhang;D. C. Scheirer;William H. Fowle;Howard M. Goodman
影响因子：
7.2
作者：
Boevink, P;Oparka, K;Hawes, C
通讯作者：
Hawes, C