How G-quadruplex topology and loop sequences affect optical properties of DNA-templated silver nanoclusters
How G-quadruplex topology and loop sequences affect optical properties of DNA-templated silver nanoclusters
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G-四链体拓扑和环序列如何影响 DNA 模板银纳米团簇的光学性质
DOI：
10.1007/s12274-017-1844-4
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发表时间：
2018-03
期刊：
影响因子：
9.9
通讯作者：
Li Na
中科院分区：
文献类型：
--
作者：
Tao Guangyu;Chen Yang;Lin Ruoyun;Zhou Jiang;Pei Xiaojing;Liu Feng;Li Na
在DNA模板化银纳米团簇（DNA-Ag NC）的制备研究中，模板如何影响纳米团簇的荧光仍不清楚，理解DNA模板和银纳米团簇性质之间的相关性一直是一个挑战。在这方面，基于一系列结构确定的分子内G-四链体的合理设计，我们制备了G-四链体模板化的Ag NC，其中确定的G-四链体与银的比例为1：2。我们使用圆二色谱、消光和发射光谱评估了G-四链体拓扑结构和环序列对DNA-Ag NC荧光的影响。发现具有反平行拓扑结构的G-四链体模板与平行和混合配置相比产生具有更强荧光的Ag NCs。环碱基相邻的G-四分体有一个更显着的影响相比，那些在中间的环，腺嘌呤主要表现出增强效应和胸腺嘧啶是有害的银NC的荧光。一般来说，具有反平行拓扑结构的G-四链体与腺嘌呤在环相邻的G-四联体将是良好的模板，用于生产高荧光Ag NC。这是第一个研究集中在G-四链体拓扑结构/序列和银纳米粒子的光学性质之间的相关性。我们希望本研究的结果将有助于更深入地了解G-四链体模板和Ag NCs之间的相关性，并有助于理解和利用其独特的属性。
In the study of the fabrication of DNA-templated silver nanoclusters (DNA-Ag NCs), how templates affect the fluorescence of the nanoclusters remains unclear, and it has been a challenge to understand the correlation between the properties of the DNA template and the Ag NCs. In this respect, based on the rational design of a series of structurally defined intramolecular G-quadruplexes, we prepared G-quadruplex-templated Ag NCs with a defined G-tetrad-to-silver ratio of 1:2. We evaluated the effect of G-quadruplex topology and loop sequences on the fluorescence of DNA-Ag NCs using circular dichroism, and extinction and emission spectroscopy. G-quadruplex templates with an anti-parallel topology were found to produce Ag NCs with stronger fluorescence compared with parallel and hybrid configurations. Loop bases adjacent to G-tetrads have a more significant impact on the fluorescence of Ag NCs compared with those in the middle of the loop, with adenine largely exhibiting an enhancement effect and thymine being detrimental. Generally, G-quadruplexes having an anti-parallel topology with adenine in the loop adjacent to the G-tetrad would be good templates for producing highly fluorescent Ag NCs. This is the first study to focus on the correlation between G-quadruplex topology/sequence and the optical properties of Ag NCs. We hope that the results of this study will facilitate a more in-depth understanding of correlation between G-quadruplex templates and Ag NCs, and help to understand and utilize their unique attributes.
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DNA稳定的荧光银簇中的魔术数字导致魔术色。
DOI：
10.1021/jz500146q
发表时间：
2014-03-20
期刊：
The journal of physical chemistry letters
影响因子：
--
作者：
Copp SM;Schultz D;Swasey S;Pavlovich J;Debord M;Chiu A;Olsson K;Gwinn E
通讯作者：
Gwinn E
影响因子：
29.4
作者：
Stacy M. Copp;Petko Bogdanov;M. Debord;Ambuj K. Singh;E. Gwinn
通讯作者：
Stacy M. Copp;Petko Bogdanov;M. Debord;Ambuj K. Singh;E. Gwinn
影响因子：
6.7
作者：
Sharma, Jaswinder;Rocha, Reginaldo C.;Martinez, Jennifer S.
通讯作者：
Martinez, Jennifer S.
影响因子：
29.4
作者：
Gwinn, Elisabeth G.;O'Neill, Patrick;Fygenson, Deborah Kuchnir
通讯作者：
Fygenson, Deborah Kuchnir
DOI：
10.1073/pnas.0610677104
发表时间：
2007-07-31
影响因子：
11.1
作者：
Vosch, Tom;Antoku, Yasuko;Dickson, Robert M.
通讯作者：
Dickson, Robert M.