Blocked synthesis of sporopollenin and jasmonic acid leads to pollen wall defects and anther indehiscence in genic male sterile wheat line 4110S at high temperatures
Blocked synthesis of sporopollenin and jasmonic acid leads to pollen wall defects and anther indehiscence in genic male sterile wheat line 4110S at high temperatures
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高温下孢粉素和茉莉酸合成受阻导致小麦雄性不育系4110S花粉壁缺陷和花药不裂
DOI：
10.1007/s10142-019-00722-y
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发表时间：
2019-11
影响因子：
2.9
通讯作者：
Song Xiyue
中科院分区：
文献类型：
--
作者：
Yang Xuetong;Ye Jiali;Zhang Lingli;Song Xiyue
环境敏感核雄性不育是杂交种生产和育种的有效工具，但育性转换的分子机制尚未见报道。本研究采用RNA-seq、表型和细胞学观察以及生理指标分析等方法，对温敏核不育系4110 S在不同温度条件下的育性转换机制进行了研究。总共鉴定了3420个差异表达基因（DEG），包括2331个上调基因和1089个下调基因。DEG明显分布在54个基因本体功能组中。苯丙素、长链脂肪酸和茉莉酸（JA）生物合成途径与雄性不育有关，它们的下调阻断了孢粉素和JA的合成。表型和细胞学分析表明，花粉壁缺陷和花药在高温下开裂诱导不育。此外，酶联免疫吸附试验结果表明，JA的丰度在限制性条件下（高温）比允许条件下（低温）的4110 S低。一个可能的调节网络的途径与雄性不育的建议。这些结果为温敏雄性不育系统育性转换的分子机制提供了新的认识。
Environment-sensitive genic male sterility is a valid tool for hybrid production and hybrid breeding, but there are no previous reports of the molecular mechanism of fertility conversion. In this study, RNA-seq, phenotypic and cytological observations, and physiological indexes were applied to analyze thermo-sensitive genic male sterility line 4110S under different temperature conditions to explore the fertility transformation mechanism. In total, 3420 differentially expressed genes (DEGs) were identified comprising 2331 upregulated genes and 1089 downregulated genes. The DEGs were apparently distributed among 54 Gene Ontology functional groups. The phenylpropanoid, long-chain fatty acid, and jasmonic acid (JA) biosynthesis pathways were related to male sterility, where their downregulation blocked the synthesis of sporopollenin and JA. Phenotypic and cytological analyses showed that pollen wall defects and anther indehiscence at high temperatures induced sterility. Moreover, enzyme-linked immunosorbent assay results indicated that the abundance of JA was lower in 4110S under restrictive conditions (high temperature) than permissive conditions (low temperature). A possible regulated network of pathways associated with male sterility was suggested. These results provided insights into the molecular mechanism of fertility conversion in the thermosensitive male sterility system.
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DOI：
--
发表时间：
2010
期刊：
Journal of Triticeae Crops
影响因子：
--
作者：
Li You-yong
通讯作者：
Li You-yong
DOI：
--
发表时间：
2006
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
L. Gong
通讯作者：
L. Gong
DOI：
--
发表时间：
2005
期刊：
Journal of Northwest Forestry University
影响因子：
--
作者：
Du Li-na;Zhu Wei
通讯作者：
Du Li-na;Zhu Wei
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
11.6
作者：
McConn, M;Browse, J
通讯作者：
Browse, J