A protein kinase C inhibitory activity is present in rat brain homogenate
A protein kinase C inhibitory activity is present in rat brain homogenate
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大鼠脑匀浆中存在蛋白激酶 C 抑制活性
DOI：
10.1016/0014-5793(84)80976-x
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
C. Le Peuch
中科院分区：
文献类型：
--
作者：
Nicole Schwantke;C. Le Peuch
The partial purification and characterization of (a) factor(s) from rat brain which inhibit(s) the activity of calcium and phospholipid‐dependent protein kinase from the same tissue is described. This factor, present in 100000 ×grat brain homogenate supernatant, is inactivated upon treatment by trypsin and pepsin and is therefore assumed to be a protein. It was partially purified by ion‐exchange chromatography on DEAEcellulose, ammonium sulfate precipitation and gel filtration. This inhibitor is not stable to heating at 70°C for 10 min, however partial renaturation of the inhibitory activity can be observed after incubation of the denatured inhibitor for 24 h at 4°C. It is precipitable by 10% trichloroacetic acid and by 2 M ammonium sulfate. It exhibits a Stokes radius of 20 Å by gel exclusion chromatography, corresponding to a molecular mass of 20 kDa assuming a globular shape. Kinetic analysis of the inhibition of calcium‐phospholipid‐dependent histone kinase activity indicates that the inhibitor is competitive with respect to the protein substrate. No change was observed in the kinetic values of the kinase for ATP, Ca2+and phospholipids.
纯化的大鼠脑钙和磷脂依赖性蛋白激酶磷酸化核糖体蛋白 S6。
DOI：
10.1073/pnas.80.22.6858
发表时间：
1983
影响因子：
11.1
作者：
LePeuch,CJ;Ballester,R;Rosen,OM
通讯作者：
Rosen,OM
来自心脏的磷脂敏感 Ca2 依赖性蛋白激酶。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wise,BC;Raynor,RL;Kuo,JF
通讯作者：
Kuo,JF
DOI：
10.1073/pnas.81.7.2117
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SUGIMOTO, Y;WHITMAN, M;ERIKSON, RL
通讯作者：
ERIKSON, RL