Genome‑wide analysis and prediction of functional long noncoding RNAs in osteoblast differentiation under simulated microgravity.
Genome‑wide analysis and prediction of functional long noncoding RNAs in osteoblast differentiation under simulated microgravity.
复制标题
模拟微重力下成骨细胞分化中功能性长非编码RNA的全基因组分析和预测。
DOI：
10.3892/mmr.2017.7671
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发表时间：
2017-12
影响因子：
3.4
通讯作者：
Cao X
中科院分区：
文献类型：
--
作者：
Hu Z;Wang H;Wang Y;Zhou H;Shi F;Zhao J;Zhang S;Cao X
长非编码RNA(LncRNAs)被认为是细胞发育过程中许多生物学过程中的重要调节因子。然而，在模拟微重力条件下成骨细胞分化过程中整体lncRNA的表达模式和潜在功能仍不清楚。本研究采用高通量微阵列技术检测了MC3TC-E1前成骨细胞在模拟微重力条件下成骨分化过程中的lncRNA和mRNA表达谱。当MC3T3-E1细胞暴露于模拟微重力时，857个lncRNAs和2,264个mRNAs的表达发生了显著变化。观察到差异表达的lncRNAs和mRNAs在染色体上有相对一致的分布模式和共表达网络。基因组上下文分析进一步鉴定了132个差异表达的LncRNA和附近的编码基因对。随后，根据它们与去调控的mRNAs的共表达关联和潜在的顺式作用调控模式，筛选出3个lncRNAs在成骨细胞分化中可能的功能。本研究旨在为进一步研究lncRNA在机械信号介导的成骨细胞分化中的作用提供全面的认识和基础。
Long noncoding RNAs (lncRNAs) have been regarded as important regulators in numerous biological processes during cell development. However, the holistic lncRNA expression pattern and potential functions during osteoblast differentiation under simulated microgravity remain unknown. In the present study, a high throughput microarray assay was performed to detect lncRNA and mRNA expression profiles during MC3TC-E1 pre-osteoblast cell osteo-differentiation under simulated microgravity. The expression of 857 lncRNAs and 2,264 mRNAs was significantly altered when MC3T3-E1 cells were exposed to simulated microgravity. A relatively consistent distribution pattern on the chromosome and a co-expression network were observed between the differentially-expressed lncRNAs and mRNAs. Genomic context analysis further identified 132 differentially-expressed lncRNAs and nearby coding gene pairs. Subsequently, 3 lncRNAs were screened out for their possible function in osteoblast differentiation, based on their co-expression association and potential cis-acting regulatory pattern with the deregulated mRNAs. The present study aimed to provide a comprehensive understanding of and a foundation for future studies into lncRNA function in mechanical signal-mediated osteoblast differentiation.
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