An observational assessment method for aging laboratory rats.
An observational assessment method for aging laboratory rats.
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衰老实验大鼠的观察评估方法。
DOI：
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发表时间：
2010
影响因子：
1.7
通讯作者：
R. Macphail
中科院分区：
文献类型：
--
作者：
P. Phillips;K. Jarema;D. Kurtz;R. Macphail
人口老龄化的快速增长凸显了对实验动物模型的需求，以研究衰老的基本生物学过程以及对疾病、药物和环境污染物的易感性。需要方法来评估衰老动物随时间的健康状况，特别是有效监测大型研究群体的方法。在这里，我们描述了一种观察评估方法，该方法可以在大约 1 分钟内完成，按 5 分制对外观、姿势、活动能力和肌肉张力进行评分。 1分表示没有恶化，而5分表示严重恶化。测试对象为 12 至 36 月龄的雄性棕色挪威大鼠 (n = 32)。这些大鼠同时参与间歇性暴露（大约每 4 个月）短效环境化学物质的行为影响实验。结果表明，在 18 个月之前，与衰老相关的恶化迹象并未出现。评估分数和变异性随着年龄的增长而增加。大约 24 个月前体重一直增加，然后保持稳定，但剩下的几只老鼠在 31 个月后体重下降。 20~28月龄死亡发生率略有上升，随后急剧上升；中位生存年龄约为 30 个月，最长为 36 个月。结果表明，我们的观察评估方法支持有效监测衰老大鼠的健康状况，并可能有助于研究老年期间对疾病、药物和毒物的易感性。
The rapid growth of the aging human population highlights the need for laboratory animal models to study the basic biologic processes of aging and susceptibility to disease, drugs, and environmental pollutants. Methods are needed to evaluate the health of aging animals over time, particularly methods for efficiently monitoring large research colonies. Here we describe an observational assessment method that scores appearance, posture, mobility, and muscle tone on a 5-point scale that can be completed in about 1 min. A score of 1 indicates no deterioration, whereas a score of 5 indicates severe deterioration. Tests were applied to male Brown Norway rats between 12 and 36 mo of age (n = 32). The rats were participating concurrently in experiments on the behavioral effects of intermittent exposure (approximately every 4 mo) to short-acting environmental chemicals. Results demonstrated that aging-related signs of deterioration did not appear before 18 mo of age. Assessment scores and variability then increased with age. Body weights increased until approximately 24 mo, then remained stable, but decreased after 31 mo for the few remaining rats. The incidence of death increased slightly from 20 to 28 mo of age and then rose sharply; median survival age was approximately 30 mo, with a maximum of 36 mo. The results indicate that our observational assessment method supports efficient monitoring of the health of aging rats and may be useful in studies on susceptibility to diseases, drugs, and toxicants during old age.
DOI：
10.1093/gerona/63.4.391
发表时间：
2008-04-01
影响因子：
5.1
作者：
Walston, Jeremy;Fedarko, Neal;Becker, Kevin
通讯作者：
Becker, Kevin
个体间对环境毒物的易感性——当前评估。
影响因子：
3.8
作者：
Nebert,DanielW
通讯作者：
Nebert,DanielW