A fiber optoacoustic emitter with controlled ultrasound frequency for cell membrane sonoporation at submillimeter spatial resolution.
A fiber optoacoustic emitter with controlled ultrasound frequency for cell membrane sonoporation at submillimeter spatial resolution.
复制标题
DOI：
10.1016/j.pacs.2020.100208
复制
发表时间：
2020-12
期刊：
影响因子：
7.9
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Shi L;Jiang Y;Zhang Y;Lan L;Huang Y;Cheng JX;Yang C
研制了一种基于光纤的光声发射器，作为迄今为止最小型化的亚毫米级超声点源。实现了可控频率，并进一步诱导了频率相关效率的细胞膜声穿孔。通过突破衍射极限，解决了亚mhz频率和亚毫米精度之间的折衷问题，在区域特异性小区调制中显示出巨大的潜力。聚焦超声在微创治疗和机制研究中备受关注。低于1mhz的频率被认为是高效生物调制的优选。然而，典型的亚mhz超声技术的空间约束较差，仅为几毫米，器件直径大到~ 25毫米，受到衍射极限的限制，严重阻碍了其进一步的应用。为了解决这一问题，开发了一种基于光纤的光声发射器（FOE），作为亚毫米约束的小型化超声点源，由光纤上的光扩散层和膨胀层组成。通过改变声阻尼和光吸收性能，可以实现0.083 MHz - 5.500 MHz范围内的可控频率，并进一步诱导具有频率依赖效率的细胞膜声穿孔。通过突破衍射极限，解决了亚mhz频率和亚毫米精度之间的折衷问题，在区域特异性药物传递、基因转染和神经刺激方面显示出巨大的潜力。
A fiber-based optoacoustic emitter is developed, serving as the most miniaturized ultrasound point source so far, with sub-millimeter confinement. Controllable frequencies are achieved and further induce cell membrane sonoporation with frequency dependent efficiency. By solving the problem of compromise between sub-MHz frequency and sub-millimeter precision via breaking the diffraction limit, the FOE shows a great potential in region-specific cell modulation. Focused ultrasound has attracted great attention in minimally invasive therapeutic and mechanism studies. Frequency below 1 MHz is identified preferable for high-efficiency bio-modulation. However, the poor spatial confinement of several millimeters and large device diameter of ∼25 mm of typical sub-MHz ultrasound technology suffered from the diffraction limit, severely hindering its further applications. To address it, a fiber-based optoacoustic emitter (FOE) is developed, serving as a miniaturized ultrasound point source, with sub-millimeter confinement, composed of an optical diffusion layer and an expansion layer on an optical fiber. By modifying acoustic damping and light absorption performance, controllable frequencies in the range of 0.083 MHz–5.500 MHz are achieved and further induce cell membrane sonoporation with frequency dependent efficiency. By solving the problem of compromise between sub-MHz frequency and sub-millimeter precision via breaking the diffraction limit, the FOE shows a great potential in region-specific drug delivery, gene transfection and neurostimulation.
登录
查看更多内容
影响因子：
14
作者：
Mulik RS;Bing C;Ladouceur-Wodzak M;Munaweera I;Chopra R;Corbin IR
通讯作者：
Corbin IR
影响因子：
4
作者：
Mohammadzadeh, M.;Gonzalez-Avila, S. R.;Ohl, C. D.
通讯作者：
Ohl, C. D.
影响因子：
2.9
作者：
Karshafian, Raffi;Bevan, Peter D.;Burns, Peter N.
通讯作者：
Burns, Peter N.
影响因子：
2.4
作者：
Ferraro, Giuseppe A.;De Francesco, Francesco;D'Andrea, Francesco
通讯作者：
D'Andrea, Francesco
DOI：
10.1109/t-su.1978.31001
发表时间：
1978-01-01
期刊：
IEEE TRANSACTIONS ON SONICS AND ULTRASONICS
影响因子：
--
作者：
DESILETS, CS;FRASER, JD;KINO, GS
通讯作者：
KINO, GS