Loss of Binding Capabilities in an Ecologically Important Odorant Receptor of the Fall Armyworm, Spodoptera frugiperda, by a Single Point Mutation.
Loss of Binding Capabilities in an Ecologically Important Odorant Receptor of the Fall Armyworm, Spodoptera frugiperda, by a Single Point Mutation.
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单点突变导致秋粘虫、草地贪夜蛾的生态重要气味受体失去结合能力。
DOI：
10.1021/acs.jafc.3c04247
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发表时间：
2023
影响因子：
6.1
通讯作者：
Yongjun Zhang
中科院分区：
文献类型：
--
作者：
Xiaohe Liu;Long;A. Khashaveh;S. Shan;Beibei Lv;S. Gu;Yongjun Zhang
在秋粘虫（Spodoptera frugiperda，鳞翅目：夜蛾科）中，嗅觉在寻找食物来源、配偶和产卵地点方面起着至关重要的作用。本研究新发现鳞翅目中高度保守的气味受体SfruOR14。在双电极电压钳记录中，SfruOR14/Orco配合物被狭窄地调谐到六种挥发性化合物，包括苯乙醛（PAA）、苯甲醛、七醛、(E)-2-己烯-1-al、肉桂醛和2-苯乙醇，其中PAA表现出最强的结合亲和力。随后的同源性建模和分子对接显示，SfruOR14中的Phe79、His83、Tyr149、Pro176、Gln177、Leu202和Thr348是这6个配体的关键结合残基。最后，由于定点突变，SfruOR14His83Ala突变体完全失去了对所有配体的结合能力。综上所述，我们的研究结果为理解SfruOR14与包括PAA在内的化学配体之间的相互作用提供了有价值的见解，这有助于设计用于害虫防治的新型嗅觉调节剂。
Olfaction plays a crucial role in locating food sources, mates, and spawning sites in the fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae). In the current study, SfruOR14, a highly conserved odorant receptor (OR) in lepidopteran species, was newly uncovered in S. frugiperda. In two-electrode voltage clamp recordings, the SfruOR14/Orco complex was narrowly tuned to six volatile compounds including phenylacetaldehyde (PAA), benzaldehyde, heptaldehyde, (E)-2-hexen-1-al, cinnamaldehyde, and 2-phenylethanol, among which PAA showed the strongest binding affinity. Subsequent homology modeling and molecular docking revealed that Phe79, His83, Tyr149, Pro176, Gln177, Leu202, and Thr348 in SfruOR14 were the key binding residues against the six ligands. Finally, as a result of site-directed mutagenesis, the SfruOR14His83Ala mutant completely lost its binding capabilities toward all ligands. Taken together, our findings provide valuable insights into understanding the interaction between SfruOR14 and the chemical ligands including PAA, which can help to design novel olfactory modulators for pest control.
DOI：
10.1073/pnas.1907847116
发表时间：
2019-11-05
影响因子：
11.1
作者：
Kawahara, Akito Y.;Plotkin, David;Breinholt, Jesse W.
通讯作者：
Breinholt, Jesse W.
影响因子：
3.5
作者：
Hughes, David T.;Wang, Guirong;Luetje, Charles W.
通讯作者：
Luetje, Charles W.