Metabolome Analysis under Aluminum Toxicity between Aluminum-Tolerant and -Sensitive Rice (Oryza sativa L.).
Metabolome Analysis under Aluminum Toxicity between Aluminum-Tolerant and -Sensitive Rice (Oryza sativa L.).
复制标题
铝耐受和敏感水稻（Oryza sativa L.）铝毒性代谢组分析
DOI：
10.3390/plants11131717
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发表时间：
2022-06-28
期刊：
影响因子：
4.5
通讯作者：
Zhang, Peng
中科院分区：
文献类型：
--
作者：
Xie, Lihua;Li, Huijuan;Zhong, Zhengzheng;Guo, Junjie;Hu, Guocheng;Gao, Yu;Tong, Zhihua;Liu, Meilan;Hu, Songping;Tong, Hanhua;Zhang, Peng
关键词：
铝（Al）在酸性土壤中溶解成三价离子（Al 3+），抑制根系生长。由于全球约13%的水稻种植在酸性土壤上，提高水稻的耐铝性可能会显着提高产量。本研究对耐铝品种日本晴和铝敏感品种H570进行了铝毒胁迫下的代谢组学分析。在铝毒条件下，分别在日本晴和H570中检测到45和83种差异代谢产物。此外，结果显示，在日本晴中，45种总代谢产物中有16种脂质表达下调，45种代谢产物中有7种酚酸和4种生物碱表达上调，而在H570中特异性检测到12种氨基酸及其衍生物，其中11种氨基酸表达上调，包括参与半胱氨酸合成的L-高丝氨酸和L-蛋氨酸，与腐胺合成相关的L-鸟氨酸和L-脯氨酸，以及与乙烯合成相关的1-氨基环丙烷-1-羧酸。水稻中与铝解毒有关的半胱氨酸和S-（甲基）谷胱甘肽含量显著降低。同时，腐胺在H570中有积累，而在日本晴中变化不明显，推测其可能是水稻解毒铝的中间产物。本研究中检测到的耐铝和敏感水稻变异体之间的差异代谢产物可能在耐铝性中起重要作用。这些结果为水稻耐铝机制的研究提供了新的思路。
Aluminum (Al) solubilizes into trivalent ions (Al3+) on acidic soils, inhibiting root growth. Since about 13% of global rice cultivation is grown on acidic soils, improving Al tolerance in rice may significantly increase yields. In the present study, metabolome analysis under Al toxicity between the Al-tolerant variety Nipponbare and the Al-sensitive variety H570 were performed. There were 45 and 83 differential metabolites which were specifically detected in Nipponbare and H570 under Al toxicity, respectively. Furthermore, the results showed that 16 lipids out of 45 total metabolites were down-regulated, and 7 phenolic acids as well as 4 alkaloids of 45 metabolites were up-regulated in Nipponbare, while 12 amino acids and their derivatives were specifically detected in H570, of which 11 amino acids increased, including L-homoserine and L-methionine, which are involved in cysteine synthesis, L-ornithine and L-proline, which are associated with putrescine synthesis, and 1-aminocyclopropane-1-carboxylate, which is associated with ethylene synthesis. The contents of cysteine and s-(methyl) glutathione, which were reported to be related to Al detoxification in rice, decreased significantly. Meanwhile, putrescine was accumulated in H570, while there was no significant change in Nipponbare, so we speculated that it might be an intermediate product of Al detoxification in rice. The differential metabolites detected between Al-tolerant and -sensitive rice variants in the present study might play important roles in Al tolerance. These results provide new insights in the mechanisms of Al tolerance in rice.
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水稻ASR1和ASR5是调节铝反应基因的互补转录因子。
DOI：
10.1111/pce.12655
发表时间：
2016-03
期刊：
Plant, cell & environment
影响因子：
--
作者：
Arenhart RA;Schunemann M;Bucker Neto L;Margis R;Wang ZY;Margis-Pinheiro M
通讯作者：
Margis-Pinheiro M
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影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
4.3
作者：
Larsen, Paul B.;Cancel, Jesse;Ochoa, Vanessa
通讯作者：
Ochoa, Vanessa
影响因子：
7.2
作者：
Huang, Chao-Feng;Yamaji, Naoki;Ma, Jian Feng
通讯作者：
Ma, Jian Feng
影响因子：
7.4
作者：
Chen, Zhi Chang;Yamaji, Naoki;Ma, Jian Feng
通讯作者：
Ma, Jian Feng