Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates.
Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates.
复制标题
非人类灵长类动物的埃博拉病毒出血热的疫苗接种。
DOI：
10.1038/nature01876
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发表时间：
2003-08-07
期刊：
影响因子：
64.8
通讯作者：
Nabel GJ
中科院分区：
文献类型：
--
作者：
Sullivan NJ;Geisbert TW;Geisbert JB;Xu L;Yang ZY;Roederer M;Koup RA;Jahrling PB;Nabel GJ
遏制高致命性埃博拉病毒爆发对公共卫生构成严重挑战。尽管一种实验性疫苗成功地保护了非人类灵长类动物免受疾病的侵害，但完成免疫接种需要六个多月的时间，这使得限制急性流行病变得不切实际。在这里，我们报告了在非人类灵长类动物中加速接种埃博拉病毒疫苗的进展。用编码埃博拉糖蛋白（GP）的腺病毒（ADV）载体免疫的抗体应答比用DNA引发和ADV加强更快地诱导，但其幅度较低。为了确定这种早期的免疫应答是否仍然可以防止疾病，在用ADV-GP和核蛋白（NP）载体接种疫苗后，用埃博拉病毒攻击食蟹猴。保护是非常有效的，并与埃博拉特异性CD 8 + T细胞和抗体反应的产生相关。即使用ADV-GP/NP免疫动物一次并在28天后攻击，它们仍然对低剂量或高剂量的病毒攻击具有抗性。这种加速疫苗提供了一种干预措施，可能有助于限制埃博拉病毒的流行传播，并适用于其他病毒。本文的在线版本（doi：10.1038/nature 01876）包含补充材料，可供授权用户使用。
Containment of highly lethal Ebola virus outbreaks poses a serious public health challenge. Although an experimental vaccine has successfully protected non-human primates against disease, more than six months was required to complete the immunizations, making it impractical to limit an acute epidemic. Here, we report the development of accelerated vaccination against Ebola virus in non-human primates. The antibody response to immunization with an adenoviral (ADV) vector encoding the Ebola glycoprotein (GP) was induced more rapidly than with DNA priming and ADV boosting, but it was of lower magnitude. To determine whether this earlier immune response could nonetheless protect against disease, cynomolgus macaques were challenged with Ebola virus after vaccination with ADV–GP and nucleoprotein (NP) vectors. Protection was highly effective and correlated with the generation of Ebola-specific CD8+ T-cell and antibody responses. Even when animals were immunized once with ADV–GP/NP and challenged 28 days later, they remained resistant to challenge with either low or high doses of virus. This accelerated vaccine provides an intervention that may help to limit the epidemic spread of Ebola, and is applicable to other viruses. The online version of this article (doi:10.1038/nature01876) contains supplementary material, which is available to authorized users.
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