Shear-induced changes in endothelin-1 secretion of microvascular endothelial cells.
Shear-induced changes in endothelin-1 secretion of microvascular endothelial cells.
复制标题
剪切诱导的微血管内皮细胞内皮素-1 分泌的变化。
DOI：
10.1006/mvre.2001.2387
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发表时间：
2002
影响因子：
3.1
通讯作者：
S. Xu
中科院分区：
文献类型：
--
作者：
G.;S. Cai;Ping Wang;K. Ouyang;Y;S. Xu
人肾小球微血管内皮细胞（HGMEC）培养单层在静态培养中作为对照，或承受0.5、1.0或1.5 N/m2的稳定层流剪切应力。剪切25 h时，对照组ET-1的累积分泌量为705.4 pg/cm2, 0.5 N/m2时为820.7 pg/cm2, 1.0 N/m2时为1063.2 pg/cm2, 1.5 N/m2时为644.7 pg/cm2。在0.5、1.0和1.5 N/m2剪切应力下，HGMEC单层膜的ET-1平均分泌率分别为32.83 +/- 2.01 pg/cm2 x h、42.53 +/- 3.74 pg/cm2 x h和25.79 +/- 1.29 pg/cm2 x h。静态对照的平均ET-1分泌速率为28.22 +/- 3.11 pg/cm2 x h。结果表明，低剪切应力（0.5 N/m2）升高了ET-1的分泌，高剪切应力（1.5 N/m2）抑制了ET-1的分泌，而中等剪切应力（1.0 N/m2）使ET-1的分泌达到最大值，且ET-1的分泌随剪切持续时间呈非线性变化。并且logistic方程可以用来描述剪切持续时间与ET-1分泌之间的关系。ET-1的主要分泌周期在5.3 ~ 22.3 h之间，峰值分泌速率约在10.7 ~ 15.2 h之间。我们的研究结果还表明，HGMECs的主要分泌周期和峰值分泌速率随剪切应力水平的变化而变化。因此，培养的人微血管内皮细胞对剪切应力的反应在ET-1分泌方面与培养的大血管内皮细胞不同。除了剪切应力水平外，剪切持续时间也是ET-1分泌的重要决定因素。因此，在未来血管内皮细胞培养物分泌的研究中，应考虑血管内皮细胞的异质性和剪切的持续时间。
Human glomerular microvascular endothelial cell (HGMEC) culture monolayers were maintained in static culture as controls or subjected to steady laminar shear stress of 0.5, 1.0, or 1.5 N/m2. Over 25 h of shear, the cumulative secretion of ET-1 was 705.4 pg/cm2 in the control, 820.7 pg/cm2 at 0.5 N/m2, 1063.2 pg/cm2 at 1.0 N/m2, and 644.7 pg/cm2 at 1.5 N/m2. The average ET-1 secretion rate for the HGMEC monolayers exposed to 0.5, 1.0, or 1.5 N/m2 of shear stress was 32.83 +/- 2.01 pg/cm2 x h, 42.53 +/- 3.74 pg/cm2 x h, and 25.79 +/- 1.29 pg/cm2 x h, respectively. The average ET-1 secretion rate of the static controls was 28.22 +/- 3.11 pg/cm2 x h. The results showed that low shear stress (0.5 N/m2) elevated and high shear stress (1.5 N/m2) suppressed secretion of ET-1, while an intermediate level of shear stress (1.0 N/m2) led to the maximum secretion of ET-1, and furthermore, ET-1 secretion varied with the duration of shear in a nonlinear fashion, and the logistic equations may be used to describe relationship between the duration of shear and the ET-1 secretion. The major secretion period of ET-1 occurred between 5.3 and 22.3 h, with the peak secretion rate occurring at approximately 10.7-15.2 h. Our findings showed also that the major secretion period and peak secretion rate of HGMECs varied with the level of shear stress. Thus, the response of cultured human microvascular endothelial cells to shear stress differed from that of large-vessel endothelial cell cultures in terms of ET-1 secretion. In addition to the level of shear stress, the duration of shear is an important determinant in ET-1 secretion. Consequently, the heterogeneity of vascular endothelial cells and the duration of shear should both be considered in future research on the secretion of vascular endothelial cell cultures.
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影响因子：
3.2
作者：
Boegehold,MA
通讯作者：
Boegehold,MA
DOI：
--
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Shubeita;Patrick M. McDonough;A. N. Harris;Kirk U. Knowlton;C. C. Glembotski-C.;Joan Heller Brown;Kenneth R. Chien
通讯作者：
H. Shubeita;Patrick M. McDonough;A. N. Harris;Kirk U. Knowlton;C. C. Glembotski-C.;Joan Heller Brown;Kenneth R. Chien
影响因子：
4.3
作者：
John B. Sharefkin;Scott L. Diamond;S. Eskin;L. McIntire;Carl W. Dieffenbach
通讯作者：
John B. Sharefkin;Scott L. Diamond;S. Eskin;L. McIntire;Carl W. Dieffenbach
内皮素是成纤维细胞和平滑肌细胞的一种有效的新型激动剂，刺激磷脂酰肌醇水解、二酰甘油释放和基因表达。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Muldoon,LL;Rodland,KD;Forsythe,ML;Magun,BE
通讯作者：
Magun,BE